| TFome pUT1176 | |
|---|---|
| Template | EE292199 |
| Genbank Insert ID | HQ858717 |
| Protein Name | ZmNAC110 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G167018 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | c.start+21 functional domain still present. |
| Request Information | Deposited to ABRC |
TFome clone pUT1176
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C39 UP |
| Temperature (ºC) | 62.0 |
| Sequence
|
caccATGGAGGCGAGAATGCCT |
3' Primer
| Name | C39 DN |
| Temperature (ºC) | |
| Sequence
|
GTCCAATAACGGATAATCTaatc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MEARMPPGFRFHPRDDELVMDYLLQKLSGHGHHAGAAIVVDVDLNKCEPWDLPDSACVGGKEWYFFSLRDRKYATGQRTNRATHSGYWKATGKDRAVVAGGEVAVGMRKTLVFYRGRAPRGKKTEWVMHEFRLHPHAAPCLLAAAAPNKEDWVLCRVFYKSRTTSPRPESEDARDGTPSAEPQLPAALPLAPLADTYAAPTVAEQVYCFSGLPALPFRRPVSLGDLLEFDASEKESVTTAMTSVSNNTSSVLELAPNCNWNQENGMSRMWSPLGI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGCGAGAATGCCTCCGGGGTTCAGGTTCCACCCCAGGGATGACGAGCTCGTGATGGACTACCTCCTACAAAAGCTCTCCGGCCATGGCCACCATGCCGGCGCTGCCATCGTCGTCGACGTCGACCTCAACAAGTGCGAGCCATGGGACCTCCCAGATTCCGCGTGCGTGGGTGGGAAGGAGTGGTACTTCTTCAGCCTGCGCGACCGCAAGTACGCGACGGGGCAGCGCACTAACCGCGCCACGCACTCCGGCTACTGGAAGGCCACCGGCAAGGACCGCGCCGTCGTCGCCGGCGGCGAGGTGGCGGTGGGGATGCGCAAGACGCTGGTCTTCTACCGGGGCCGCGCGCCCAGGGGGAAGAAGACGGAGTGGGTCATGCACGAGTTCCGCCTCCACCCCCATGCCGCGCCGTGCCTGCTAGCAGCAGCTGCGCCAAACAAGGAGGACTGGGTGCTATGCAGGGTGTTCTACAAGAGCAGAACAACCAGCCCAAGGCCAGAATCTGAAGACGCCCGGGACGGCACCCCATCAGCTGAACCGCAACTGCCGGCTGCCCTGCCGCTTGCGCCCCTCGCTGACACCTACGCCGCCCCAACGGTTGCAGAGCAGGTGTACTGCTTCTCCGGTCTGCCGGCACTGCCATTCAGAAGACCAGTGAGCCTTGGGGACCTTCTGGAGTTCGACGCCTCCGAGAAGGAATCTGTCACGACAGCGATGACCAGTGTCTCGAACAACACTAGTTCAGTGCTGGAGCTGGCTCCAAATTGCAACTGGAACCAGGAAAATGGCATGTCACGGATGTGGAGCCCCCTTGGGATA
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