TFome clone pUT1178

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B45UP
Temperature (ºC)	65.0
Sequence Copy to clipboard	CAC CAT GGA GGA GGA GCG CTG C

3' Primer

Name	B45 DN
Temperature (ºC)
Sequence Copy to clipboard	GCTCCTGGCGCCGGCTGC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MEEERCFNNWDLDAVVRLGCRRRLSPPRQPDPFASLLPPTLPSPSPSPPQKEKPVVPALAKGPEPEPYAGWRFPNLGAGGGQDGDELMRAMLAVQPSLPQPLPTTTPTPTPTLPPAAGEHRQSTVTAVDVPLPQVRPAPAARAQPSGRQVPGAVPRSKRRKNQVKKVVCHVPADGSSSDMWAWRKYGQKPIKGSPYPRGYYRCSSSKGCAARKQVERSRADPNTFILTYTGEHNHAAPTHRNSLAGTTRNKFPSSAAPPPPPPSVVVGGAGAGDAQHQQPSPSPASTSTAGLSPTTPLRTPSMEEEEMDDDDDELLVEDMEMVGEDELLFLNIDADAEADAGAPLEPMPSLFDVVDEPFLNSPWLTATSSAGEPATGAAGARS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGGAGGAGCGCTGCTTCAACAACTGGGATCTGGACGCCGTCGTCCGCCTGGGCTGCCGCCGCCGCCTCTCCCCGCCACGGCAGCCCGACCCGTTTGCTTCGCTTCTCCCTCCGACCCTGCCGTCGCCGTCGCCGTCGCCGCCGCAGAAGGAGAAGCCCGTGGTGCCAGCGCTGGCTAAAGGACCAGAGCCAGAGCCATACGCCGGGTGGCGCTTTCCTAACCTCGGTGCTGGCGGCGGGCAAGACGGCGACGAGCTCATGAGGGCCATGTTAGCAGTCCAGCCTTCCCTGCCTCAGCCTCTGCCAACGACAACGCCAACGCCAACGCCAACGCTTCCCCCGGCAGCAGGAGAGCATCGACAGTCGACTGTTACAGCCGTGGACGTGCCGCTACCCCAGGTGCGCCCCGCTCCGGCCGCCAGAGCGCAGCCGAGCGGGCGGCAGGTGCCCGGTGCCGTGCCAAGATCCAAGAGAAGGAAGAACCAGGTGAAGAAGGTGGTCTGCCACGTTCCGGCGGACGGCTCGTCGTCGGACATGTGGGCGTGGCGCAAGTACGGCCAGAAGCCCATCAAGGGCTCCCCCTACCCAAGGGGATACTACCGGTGCAGCAGCTCCAAGGGGTGTGCGGCGCGGAAGCAGGTCGAGCGCAGCCGCGCGGACCCCAACACCTTCATCCTCACCTACACCGGCGAGCACAACCACGCGGCGCCGACCCACCGCAACTCGCTCGCCGGCACCACCCGAAACAAGTTCCCCTCCTCGGCGGCGCCTCCGCCGCCTCCGCCGTCCGTGGTGGTGGGCGGCGCCGGTGCCGGCGACGCGCAGCATCAGCAGCCGAGCCCGAGCCCGGCGTCCACGTCGACCGCGGGGCTCTCGCCCACGACGCCGCTGCGCACGCCGTCGATGGAGGAGGAGGAGATGGACGACGACGACGACGAGCTGCTGGTGGAGGACATGGAGATGGTCGGCGAGGACGAGCTCTTGTTCCTCAACATCGACGCCGACGCCGAAGCTGACGCCGGGGCACCACTGGAGCCCATGCCCTCGCTCTTCGACGTCGTCGACGAGCCCTTCCTAAACTCCCCTTGGCTGACGGCCACCAGCAGCGCCGGCGAGCCAGCCACAGGGGCAGCCGGCGCCAGGAGC