| TFome pUT1500 | |
|---|---|
| Template | DV168421 |
| Genbank Insert ID | HQ858758 |
| Protein Name | ZmALF8 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM2G172001 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT1500
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B97 UP |
| Temperature (ºC) | 64.7 |
| Sequence
|
CAC CAT GAA CGG CGG GGG C |
3' Primer
| Name | B97 DN |
| Temperature (ºC) | |
| Sequence
|
TGCCCTGGCCCGCTTGTT |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MNGGGSGLAPNAAHTADEVFRDYKGRRAGMIKALTTDVERFFKLCDPEKENLCLYGYPDETWEVTLPAEEVPPEIPEPALGINFARDGMNEKDWLALVAVHSDSWLLSVAFYFGARFGFDRETRRRLFSLINNMPTIFEVVTGAAKKQAKEKTPNSSSKSNRPSSKVSRAESRSKAKVPQDEEESGDDDEDEEADEHNNTLCGTCGTNDSKDQFWICCDNCEKWYHGKCVKITPARAEHIKQYKCPDCTNKRARA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAACGGCGGGGGCTCTGGGCTGGCGCCCAACGCCGCGCACACCGCGGACGAGGTCTTCCGCGACTACAAAGGCCGCCGCGCCGGCATGATCAAGGCCCTCACCACTGATGTGGAGAGGTTCTTCAAACTCTGTGACCCTGAAAAGGAGAACTTGTGCCTTTATGGCTATCCCGATGAGACGTGGGAGGTGACCTTGCCAGCTGAGGAAGTTCCCCCTGAGATCCCTGAACCAGCATTAGGAATCAACTTTGCTAGGGATGGCATGAATGAGAAAGACTGGCTGGCGCTAGTTGCAGTCCACAGTGATTCCTGGTTACTGTCTGTTGCATTCTACTTTGGTGCACGCTTTGGATTTGACAGAGAAACTAGGAGGCGACTCTTTAGCCTGATAAACAACATGCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGCAACAGGCCTAGTTCAAAAGTGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAAGAGAGCGGTGATGACGATGAGGATGAGGAGGCAGACGAGCACAACAATACCTTGTGTGGAACTTGCGGAACTAACGACAGCAAGGACCAGTTCTGGATCTGTTGTGATAACTGCGAGAAGTGGTACCATGGGAAGTGTGTCAAGATCACGCCAGCTCGAGCTGAGCATATCAAGCAGTACAAGTGCCCAGACTGCACCAACAAGCGGGCCAGGGCA
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