| TFome pUT1501 | |
|---|---|
| Template | EE033875 |
| Genbank Insert ID | HQ858759 |
| Protein Name | ZmARF7 |
| Species | Zea mays |
| TF Family | ARF |
| Gene id | GRMZM2G475263 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C442T p.(=) Present in template EST c. A1337T p. H446L . EST sequence does not cover last SNP. None present in functional domains. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1501
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C56UP |
| Temperature (ºC) | 61.3 |
| Sequence
|
CAC CAT GGC GCA AGG AGC A |
3' Primer
| Name | C56DN |
| Temperature (ºC) | |
| Sequence
|
ATA GTC CCT CTT CCC ATC CTG CC |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAQGAGRDPEELFEELWRACAGPLVELPQTNERVFYFLQGHLEQLQEPTDPALLAEQIKMFQVPNKILCKVVNVELKAETETDEMYAQITLQPEPDQVDLPQLPEPPLQETSRPVVHSFCKILTPSDTSTHGGFSVLRRHANECLPALDMSMPTPTQELITKDLHGSEWRFKHIYRGQPRRHLLTTGWSTFVTSKKLIAGDAFVYLRSETGQQRVGVRRLVQKQSTMPASVISSQSMHLGVLASASHAIRTHSIFLVYYRPRLSQSQYIVRLNKYLESSKIGFDVGMRFKMSFEGDDVPIKKFSGTVVDKGDLSPQWQGSEWKTLKVKWDEATNLNGPERVSSWEIEPFDASAPAITMPVQPSMKNKRPRETAEGLDIHALEPAQEFWLSGRPEQHEKTSVSSSEPKHQVAWTSERAGYSAMSSSICQNSAVTGSWFKGFNSSGSLPSLPEISQKLFQVTSNDARVPPWPGLSAYHADEPSSKLSCNTALCSYQTEEVAPRFSNAVEEEKKEPGMFRLFGVNLINHARSSATADKTSVGAGETSARAAGSFEDSAQLSRVTKDHTHMVNGSPREIQSHQSCSGRSRIKVQMHGNDVCRAVDLGNLDGYEQLMGEVGEMFEIKDLGSKEKEEWKVTFINDENETMEVGAVPWQEFCQMVRKIVIHSIGDRGHMEACPCLGQDGKRDY
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGCAAGGAGCAGGACGGGACCCGGAGGAGCTCTTCGAGGAGCTGTGGCGCGCCTGCGCGGGGCCGCTCGTGGAGTTGCCCCAGACCAACGAAAGGGTCTTCTACTTCCTCCAGGGTCACCTAGAACAGCTGCAAGAGCCGACGGACCCGGCGCTGCTGGCTGAGCAGATCAAGATGTTCCAGGTGCCCAACAAGATCCTCTGCAAGGTCGTCAACGTCGAGCTCAAGGCCGAGACAGAAACGGACGAGATGTACGCCCAGATCACTCTGCAGCCGGAACCAGATCAAGTGGATCTACCCCAATTACCTGAACCGCCCCTGCAGGAGACGTCAAGGCCCGTGGTGCACTCCTTCTGCAAGATCCTCACACCATCCGACACCAGTACTCATGGCGGTTTCTCCGTTCTTCGGCGTCATGCAAACGAATGCCTCCCAGCATTGGATATGTCGATGCCGACCCCGACCCAAGAGCTCATCACAAAGGACCTACATGGATCTGAATGGAGGTTCAAGCACATATACAGGGGCCAACCCCGTCGGCACCTTCTGACTACTGGATGGAGCACGTTTGTCACATCAAAGAAGCTGATTGCTGGTGACGCGTTTGTCTACCTGAGGAGCGAGACCGGACAGCAGCGTGTGGGAGTGAGGCGCCTCGTGCAGAAGCAAAGCACAATGCCAGCATCTGTTATATCAAGCCAAAGCATGCATCTTGGGGTGCTTGCAAGTGCATCACACGCTATTAGGACTCATTCCATTTTCCTAGTGTATTATAGACCAAGGTTAAGCCAAAGCCAATACATCGTCAGGCTGAACAAGTACCTGGAATCTAGTAAAATTGGATTCGATGTGGGCATGAGGTTTAAGATGAGTTTTGAAGGGGATGACGTCCCTATAAAGAAGTTTTCTGGAACAGTTGTCGATAAAGGAGATCTTTCTCCACAATGGCAAGGTTCTGAATGGAAGACATTGAAGGTCAAATGGGACGAAGCCACAAACTTAAATGGCCCTGAGAGGGTTTCCTCCTGGGAAATTGAACCATTCGATGCATCCGCACCTGCCATCACCATGCCCGTGCAACCATCAATGAAAAACAAAAGGCCTAGGGAGACAGCTGAAGGCCTGGATATTCATGCACTGGAACCTGCTCAAGAATTCTGGCTCTCTGGAAGGCCTGAACAGCATGAGAAGACAAGCGTAAGCTCCAGCGAACCCAAACATCAAGTCGCTTGGACAAGTGAACGCGCAGGATACAGTGCCATGAGCAGTTCAATCTGTCAGAATTCTGCAGTAACTGGGAGCTGGTTCAAGGGCTTCAATTCCTCCGGCTCACTCCCTTCCCTGCCAGAGATCTCCCAGAAACTGTTTCAGGTCACCAGCAACGACGCGAGGGTTCCTCCTTGGCCTGGGCTCTCCGCTTATCATGCTGACGAGCCCTCTTCCAAGCTGTCCTGCAACACTGCTCTGTGCAGCTACCAGACCGAGGAGGTGGCTCCAAGATTTTCCAATGCCGTCGAAGAGGAAAAGAAGGAGCCCGGCATGTTCCGTCTGTTTGGCGTCAATTTGATTAACCATGCTAGGAGTAGTGCTACCGCTGACAAGACGAGTGTGGGAGCAGGGGAAACATCGGCGCGAGCTGCTGGTTCTTTTGAAGACTCTGCTCAGCTATCAAGAGTGACGAAGGATCATACGCACATGGTGAACGGAAGCCCCCGAGAAATCCAAAGCCACCAGAGTTGCAGTGGAAGGAGTCGTATCAAGGTTCAAATGCATGGAAATGACGTGTGCAGAGCTGTGGATTTGGGAAATCTGGATGGGTACGAGCAGCTGATGGGTGAGGTTGGAGAGATGTTTGAGATCAAAGACCTTGGCTCCAAAGAGAAAGAGGAATGGAAGGTTACTTTCATTAACGACGAGAATGAGACAATGGAAGTCGGAGCTGTTCCTTGGCAGGAGTTTTGCCAGATGGTGAGGAAGATCGTGATCCATTCCATTGGAGACAGGGGCCATATGGAGGCTTGCCCTTGCCTGGGGCAGGATGGGAAGAGGGACTAT
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