| TFome pUT1602 | |
|---|---|
| Template | DV529833 |
| Genbank Insert ID | HQ858761 |
| Protein Name | ZmEREB69 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G085964 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.92ins3. In template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1602
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B61UP |
| Temperature (ºC) | 65.5 |
| Sequence
|
caccATGTGCGGCGGAGCCATC |
3' Primer
| Name | B61DN |
| Temperature (ºC) | |
| Sequence
|
CGCCAACATGAGGTCCAC |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MCGGAIIADFVPAGARRPATDDTMSASILSGSEDHPELPLPLPAPAPGRKTAYRGIRRRPWGRWAAEIRDPRKGARVWLGTYATPEDAARAYDVAAREIRGPKAKLNFPPAVGGGARAPAPAAAKRRRKSAATEESSASSPPPPAAAAAVVVAGGGEEALRDCMSGLEAFLGLQNADEGGGVEAWDAVDLMLA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTGCGGCGGAGCCATCATCGCCGACTTCGTCCCCGCCGGCGCCCGGCGCCCGGCAACAGACGACACCATGTCCGCCTCCATCCTCTCCGGTAGCGAGGACCATCCGGAGCTGCCGCTGCCGCTGCCGGCGCCGGCGCCGGGGCGCAAGACGGCGTACCGCGGGATCCGGCGCCGCCCGTGGGGCCGGTGGGCGGCGGAGATCCGCGACCCGCGGAAGGGCGCCCGCGTGTGGCTCGGCACCTACGCCACCCCGGAGGACGCGGCGCGCGCCTACGACGTGGCGGCGCGCGAGATCCGCGGGCCCAAGGCCAAGCTCAACTTCCCGCCCGCCGTCGGCGGCGGCGCGCGCGCGCCGGCGCCGGCGGCGGCCAAGAGGCGCCGCAAGTCCGCGGCGACCGAGGAGAGCTCGGCCTCGTCTCCTCCTCCTCCGGCGGCGGCGGCGGCCGTCGTCGTGGCGGGCGGCGGGGAGGAGGCGCTGCGCGACTGCATGTCTGGGCTCGAGGCGTTCCTGGGGCTGCAAAACGCCGACGAGGGTGGCGGCGTCGAGGCGTGGGACGCCGTGGACCTCATGTTGGCG
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