| TFome pUT1682 | |
|---|---|
| Template | DV163953 |
| Genbank Insert ID | HQ858766 |
| Protein Name | ZmGRAS21 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM2G116638 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T1006C p.C336R. In functional domain. EST does not cover SNP. |
| Request Information | Deposited to ABRC |
TFome clone pUT1682
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | GRAS UP |
| Temperature (ºC) | 58.5 |
| Sequence
|
caccATGTCAGCTAGGGATTCTAATCATTC |
3' Primer
| Name | GRAS DN |
| Temperature (ºC) | |
| Sequence
|
ACA CCATGCCGAAGATACAACCAATACTCTG |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MSARDSNHSYKCSDDSQMPYYNNSAPVGENGRIHMAENSLGHHYSSSDIGSQRINNSNPQVFEAQYCTLESSSANGVYPAQSSTSSHSISPLSGSPLSQHDSHSDHTYSSPPSASCLTEVADLLIKQKELENSIVGPGLDISSDCSRSLLQAHVPVRPDNWRQLLGINGGDLMQVVIACGKAVAENDVFATELLISELGHLVSVSGDPMQRLGAYMLEGIVARLSSSGSMLYKSLKCKEPTSSELMSYMHLLYEICPFYKFGYMSANGAIAEAIKGENFVHIIDFQIAQGSQWVTLLQALAARPGGPPYIRITGIDDSNSAYARGGGLDIVGRTLRDVANSCGLPFEFNAVPAASHEVELQHLAIRHGEIIAVNFAYQLHHVPDESVSTENHRDRIIRMIKSINPRVVTLVEQESNTNTAPFFPRYMETLNYYTAMFESIDVALPRDDRRRMSAEQHCVARDIVNLIACEGAERVERHELFGKWKSRFAMAGFRPYPLSSVVNNTINTLLHTYNSYYRLEERDGVLYLGWKNRVLVVSSAWC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCAGCTAGGGATTCTAATCATTCATATAAATGTTCGGATGATTCTCAAATGCCGTACTACAATAACTCAGCACCTGTAGGAGAAAATGGTAGGATCCATATGGCAGAAAACAGTCTAGGACATCATTACTCATCCTCCGATATTGGATCACAAAGGATCAACAATTCCAACCCTCAAGTATTTGAAGCACAGTACTGCACTCTGGAATCATCCTCAGCAAATGGTGTTTATCCTGCACAAAGCTCCACATCTTCTCATAGCATCTCCCCTTTAAGTGGGAGCCCCCTGTCTCAGCATGACAGCCACTCAGACCACACATATAGTTCTCCTCCAAGTGCTTCCTGTCTAACTGAGGTTGCAGATCTCCTGATTAAACAAAAAGAATTAGAGAATTCCATTGTTGGACCTGGACTGGACATTAGTTCTGACTGCTCTCGGAGCTTGTTGCAAGCCCATGTTCCAGTGAGACCAGACAACTGGAGACAACTTCTGGGAATTAATGGAGGAGACTTGATGCAAGTAGTCATAGCATGTGGTAAGGCTGTTGCAGAGAATGATGTCTTTGCAACAGAACTGTTAATATCTGAGCTTGGTCACCTGGTATCTGTATCTGGAGATCCAATGCAACGACTTGGAGCCTATATGCTTGAAGGAATTGTTGCTAGACTTTCTTCCTCCGGCAGTATGCTATATAAATCTTTGAAATGTAAAGAACCTACAAGCTCTGAGCTCATGTCATATATGCATCTCCTTTACGAGATCTGCCCATTCTACAAGTTTGGTTACATGTCAGCAAATGGTGCCATTGCCGAGGCTATTAAGGGCGAGAACTTTGTTCACATAATTGATTTCCAAATTGCTCAGGGGAGCCAGTGGGTTACTCTGCTACAGGCCCTTGCTGCAAGACCTGGGGGGCCACCATACATTAGAATCACTGGTATAGATGACTCAAATTCTGCTTATGCCAGAGGAGGCGGGCTTGATATAGTTGGGAGGACGTTGCGTGATGTTGCTAATTCATGTGGTCTTCCTTTTGAGTTCAATGCCGTTCCAGCTGCTAGCCATGAGGTTGAGCTTCAGCATCTTGCTATAAGACATGGGGAGATTATTGCTGTGAATTTTGCCTATCAGCTACATCATGTTCCTGACGAAAGTGTAAGTACAGAAAATCATCGGGATAGGATTATAAGAATGATCAAGAGCATCAATCCTAGGGTTGTTACTCTCGTTGAACAGGAGTCAAATACAAACACAGCTCCATTCTTCCCAAGGTACATGGAAACTCTCAACTACTATACAGCCATGTTTGAGTCGATAGATGTTGCTCTCCCAAGGGATGATAGGAGGCGGATGAGCGCAGAACAACACTGTGTTGCAAGGGATATTGTTAATTTAATCGCATGCGAGGGTGCTGAAAGGGTTGAGAGGCACGAGCTGTTTGGAAAATGGAAGTCAAGATTTGCAATGGCTGGATTTAGACCGTACCCACTGAGTTCAGTTGTGAACAACACTATCAACACATTGTTGCATACCTACAACAGCTACTACAGGCTTGAGGAGAGAGATGGTGTCCTTTACCTTGGATGGAAAAACAGAGTATTGGTTGTATCTTCGGCATGGTGT
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