| TFome pUT1768 | |
|---|---|
| Template | DR827144 |
| Genbank Insert ID | HQ858782 |
| Protein Name | ZmFARL13 |
| Species | Zea mays |
| TF Family | FAR1-like |
| Gene id | GRMZM2G114461 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1768
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B177UP |
| Temperature (ºC) | 63.8 |
| Sequence
|
caccATGATGCACATGCTGGTAGCCC |
3' Primer
| Name | B177DN |
| Temperature (ºC) | |
| Sequence
|
ATGCATGCTAGAAACTGAAGCATCTTGAA |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MMHMLVAPDRGGGELQPYVAPPAEQELELLRDNADDGLEGHVRCLRCGISGNATPHMRRGPDGPRTLCNACGIAYRKGKMRRMIEAEPPIDEAALAKLVPEVGMEFVSEEKAYEFYNKYAGHVGFSVRKSTSHKSSENITKVRTFVCSREGYNRDKKSLEAKKPRLDTRIGCPARLIIKVTPECKYRVTDFKADHNHQLAPPSTMHMLRSQRILTELQPGEAELSDDSVVTPTTKATGDLVVRQVSFLRSLSLLPADYKNYLRSKRMKAMQPGDGGAILKYLQTMQMDNPSFFYTMQIDEDDKLTNFFWADQKSRDDFNYFGDVLCLDTTYKINGYGRPLALFLGVNHHKQTIIFGAAMLYDESFDSYKWLFDSFKIAMHGKQPAVALIDQSIPLSSAMAAAWPSTTQRICAWHVYQNSLKHLNHVFQGSKTFAKDFGKCVFGYEDEDEFVFSWRSMLEKYDLRHNEWLSKVFAEKEQWALAYDRHIFCADIISALQAESFSSILKKFLSPQLELLSFFKHYERAVDEHRYAELQADFQASQSYPRIPPAKMLKQTAHTYTPVVFEIFRKEFELFMDSVLFSCGEAGTTSEYKVASSEKPKEHFVRFDSSDCSCLCTCRKFEFMGIPCCHMLKVLDYRNIKELPQKYLLKRWRRTAKSANEDNEGNATNANNGSLLIVPAPPTNHHGLQSFSAMIQDASVSSMH
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGCACATGCTGGTAGCCCCGGATAGGGGCGGAGGGGAGCTGCAGCCGTACGTAGCGCCGCCCGCAGAGCAGGAGCTGGAGCTCCTCCGCGACAACGCCGATGACGGCCTCGAGGGCCACGTGCGATGCCTAAGGTGTGGAATATCCGGCAATGCGACGCCCCATATGCGCCGGGGGCCTGATGGCCCAAGAACTCTTTGCAATGCGTGTGGCATTGCTTACAGGAAGGGGAAAATGAGAAGAATGATAGAAGCTGAACCACCCATAGATGAGGCTGCTCTTGCAAAGCTGGTTCCAGAGGTAGGCATGGAATTTGTGAGTGAGGAAAAGGCATACGAATTCTACAACAAATATGCTGGGCATGTTGGCTTTAGCGTTAGGAAAAGTACATCACATAAATCTTCAGAAAATATTACCAAAGTTAGAACTTTTGTGTGCTCAAGAGAAGGCTACAACAGGGATAAAAAATCACTGGAAGCCAAGAAGCCAAGATTGGATACACGAATCGGTTGCCCAGCGAGATTGATTATAAAAGTCACACCAGAGTGCAAGTATAGAGTAACTGATTTTAAAGCAGATCACAATCATCAGCTGGCCCCACCTTCAACGATGCATATGTTAAGGTCGCAGAGGATATTGACAGAACTTCAGCCTGGAGAAGCAGAATTATCAGATGATTCTGTAGTGACACCAACAACAAAGGCAACTGGTGATCTTGTTGTGAGACAAGTCAGTTTTCTTCGGAGTTTATCTCTCCTCCCAGCTGATTACAAGAATTATCTCCGTTCGAAGCGTATGAAGGCCATGCAACCTGGTGATGGTGGAGCAATATTGAAATATTTACAGACAATGCAAATGGATAACCCCTCGTTCTTTTACACTATGCAGATCGATGAGGATGACAAACTGACAAACTTCTTTTGGGCCGACCAAAAATCGAGAGATGACTTCAACTATTTCGGTGATGTGCTCTGCTTAGACACAACATACAAAATAAACGGTTATGGTAGACCGCTTGCGTTATTCCTTGGTGTGAATCACCATAAACAAACGATTATTTTTGGTGCAGCTATGCTTTATGATGAATCATTTGATTCATACAAGTGGCTTTTTGATAGTTTTAAGATCGCCATGCATGGAAAACAGCCTGCAGTAGCTTTGATAGATCAATCTATTCCATTGAGCAGTGCAATGGCAGCCGCCTGGCCAAGTACCACTCAACGAATTTGTGCTTGGCATGTGTATCAGAATTCTCTTAAGCACCTCAATCATGTTTTCCAAGGTTCGAAGACATTCGCGAAGGACTTCGGCAAATGTGTCTTTGGCTACGAGGATGAAGATGAATTCGTGTTTTCATGGAGAAGTATGCTGGAGAAATATGATCTTAGACATAATGAATGGTTGTCTAAAGTGTTTGCTGAGAAGGAACAGTGGGCCTTGGCATATGATAGACATATATTTTGCGCTGACATCATAAGTGCACTTCAAGCCGAGAGTTTCAGTAGTATTTTGAAGAAATTCTTGAGTCCTCAACTGGAGCTGCTGTCCTTCTTCAAGCACTATGAAAGAGCAGTGGACGAACATCGCTATGCTGAGCTGCAAGCCGACTTCCAAGCTAGTCAGAGCTATCCAAGAATACCCCCAGCCAAGATGTTAAAGCAAACTGCTCATACATATACCCCGGTGGTATTTGAGATCTTTCGTAAAGAGTTTGAGCTATTCATGGACTCTGTGTTATTCAGTTGTGGGGAGGCTGGGACGACATCTGAGTACAAGGTTGCTTCATCTGAGAAACCCAAGGAGCACTTTGTTCGGTTCGATTCAAGTGATTGCTCCTGTCTGTGCACTTGTAGGAAGTTTGAGTTTATGGGTATCCCGTGCTGTCACATGTTGAAGGTGCTAGATTACAGAAATATCAAAGAGCTTCCTCAAAAATATCTGTTGAAACGGTGGAGAAGGACCGCCAAATCTGCAAATGAAGACAATGAAGGCAATGCAACAAACGCCAACAACGGGTCATTATTGATTGTCCCTGCTCCTCCTACAAATCACCATGGGCTTCAGAGCTTCAGTGCAATGATTCAAGATGCTTCAGTTTCTAGCATGCAT
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