TFome clone pUT1769

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B203UP
Temperature (ºC)	65.6
Sequence Copy to clipboard	caccATGTGCGTGGAGGAGCCGAAG

3' Primer

Name	B203DN
Temperature (ºC)
Sequence Copy to clipboard	CTGCCTTCCCAGGAGTATCC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MCVEEPKDAERFGFGDPGVEEPAKFPLAQMERVCENTTSADFRQKLSNFVPVIRSGDWSDIGGRQYMEDTHVCIPDLAKNFGFPSLDNEVVSFYGVFDGHGGKDAAHFVCDNLPRMIVEDSDFPLQLEKVVRRSFMQIDCQFAETCSLHRASSSGTTALTAMVFGRSLLVANAGDCRAVLSRCGTAVEMSMDHRPCSLSEKLRVESLGGYVDDGYLNGLLGVTRALGDWHLEGMKGAGETGGPLSADPELKMTTLTKDDEFLIIGSDGIWDVFSNQNSVDFARKRLQEHNDVKLCCREIVEEAIRRGATDNLTAVLVSFHLEAPPRIRVDRPGRVERSISAEGLNSLRILLGRQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTGCGTGGAGGAGCCGAAGGACGCCGAACGGTTTGGTTTTGGAGACCCAGGGGTCGAGGAGCCCGCTAAGTTTCCCTTGGCTCAGATGGAGAGAGTTTGTGAAAATACAACTTCTGCTGATTTCAGGCAGAAGTTAAGCAATTTTGTCCCAGTAATCCGATCGGGGGATTGGTCTGATATTGGAGGTCGCCAATACATGGAAGACACCCACGTGTGCATTCCAGACCTTGCTAAGAACTTTGGTTTCCCATCATTAGATAATGAAGTTGTTTCATTCTATGGGGTCTTTGATGGTCACGGTGGAAAAGATGCTGCCCATTTCGTTTGTGATAATTTACCAAGGATGATTGTGGAAGATTCAGATTTCCCTCTTCAGCTTGAAAAAGTTGTGAGAAGGTCCTTTATGCAGATTGATTGCCAGTTTGCTGAGACATGCTCTCTCCATAGAGCATCGTCCTCCGGTACAACTGCACTTACAGCAATGGTTTTTGGAAGGTCTCTTCTGGTCGCTAATGCCGGTGACTGTAGGGCGGTTCTTTCCCGGTGTGGCACCGCAGTCGAAATGTCCATGGACCACAGGCCTTGCAGTCTCAGCGAAAAACTGCGCGTAGAATCGCTTGGTGGCTACGTGGACGATGGCTATCTGAACGGTCTGCTAGGAGTCACCAGAGCACTTGGTGACTGGCATCTCGAGGGCATGAAAGGAGCTGGTGAGACAGGTGGTCCCCTGAGTGCTGACCCAGAGCTCAAGATGACCACGCTGACGAAGGATGACGAGTTCTTGATAATTGGCAGCGACGGGATCTGGGACGTCTTCTCGAACCAGAACTCGGTGGACTTCGCCCGGAAGCGCCTCCAGGAGCACAACGATGTGAAGTTGTGCTGCAGGGAAATCGTCGAGGAGGCGATCAGGCGGGGGGCTACTGATAACTTGACTGCAGTGCTGGTCTCGTTCCACCTGGAGGCCCCGCCTCGGATCAGAGTGGACCGTCCTGGTAGGGTGGAGAGGAGCATATCTGCTGAAGGGCTCAATAGCCTCAGGATACTCCTGGGAAGGCAG