TFome clone pUT1800

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B182UP
Temperature (ºC)	68.8
Sequence Copy to clipboard	caccATGGAGTGGACGGCCCCGAAGC

3' Primer

Name	B182DN
Temperature (ºC)
Sequence Copy to clipboard	ATTTATCTGGTTTACACCAAAGAAACTGGG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

Copy to clipboard

MEWTAPKPASSPPLLWDWGDHAATGSGSSSDAPARRGGKEREAKRARAGEDRGGGELRCQVEGCGLDLSRVKDYHRKHRVCEAHTKSPRVIVAGQERRFCQQCSRFHALSEFDQKKRSCRRRLSDHNARRRKPQPDAFTFASAKLPSTLFDDRRQISFVWNKAPVSHVRPFTSPWDSSSDLKPPYAKEISDVSTKVGTITGQVHLDKSHMFNAIPTLSHGKDEPWPMKGLDMSISASKFDGAADLQHALSLLSAGACGLPDSVHQTSCIIQFNGASENSSDLHVTHGRNSGPASCADAQHIAAQPQSQLFHFTTDTGNTVYEPSFFGVNQIN

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGGAGTGGACGGCCCCGAAGCCCGCCTCATCGCCTCCCCTCCTCTGGGACTGGGGAGACCACGCCGCCACGGGCTCGGGCTCCTCCAGCGACGCCCCGGCGCGGCGCGGCGGGAAGGAGCGGGAGGCGAAGCGTGCCAGGGCCGGCGAGGACCGCGGGGGAGGTGAGCTGAGGTGCCAGGTCGAGGGGTGCGGATTGGACCTCAGCAGGGTCAAGGACTACCACCGTAAGCACCGCGTCTGCGAGGCCCACACCAAGTCCCCCCGCGTCATCGTCGCCGGCCAGGAGCGCCGGTTCTGCCAGCAGTGCAGCCGATTCCATGCGCTGTCAGAGTTTGATCAGAAGAAGAGGAGCTGCAGGAGGCGTCTGTCTGATCACAATGCCCGCCGCCGGAAGCCTCAGCCAGATGCATTCACCTTCGCCTCTGCAAAGCTGCCTTCGACATTGTTTGATGATAGGCGGCAAATAAGTTTTGTCTGGAATAAAGCTCCTGTTAGCCATGTAAGACCCTTCACTTCTCCATGGGACAGCTCATCTGACTTAAAGCCCCCTTATGCAAAGGAAATAAGTGATGTATCAACAAAAGTTGGGACAATTACTGGACAAGTTCATTTGGATAAATCTCACATGTTCAATGCTATTCCAACACTTAGCCATGGCAAAGATGAGCCTTGGCCAATGAAAGGTCTGGACATGTCTATATCTGCTTCAAAATTCGATGGAGCAGCGGATCTTCAGCATGCTCTCTCTCTTCTGTCAGCCGGCGCTTGTGGACTGCCTGATTCTGTACATCAAACATCTTGCATTATCCAATTCAATGGTGCCAGCGAGAACAGCAGTGACCTTCATGTAACGCATGGCAGGAACTCTGGTCCAGCATCATGCGCCGATGCGCAGCATATAGCTGCCCAGCCTCAGTCTCAGCTGTTTCATTTTACCACAGATACCGGCAATACTGTTTATGAGCCCAGTTTCTTTGGTGTAAACCAGATAAAT