TFome clone pUT1801

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B195UP
Temperature (ºC)	60.9
Sequence Copy to clipboard	caccATGTCTTCGTCGGGGC

3' Primer

Name	B195DN
Temperature (ºC)
Sequence Copy to clipboard	TTCATCAATAGCACTATTAGAAACATCTGGG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MSSSGPLPATGTHHGSQRAECKSQGSTSARKVQKADREKMRRDKLNEQFQDLGNALDPDRPRNDKATILGDTIQMLKDLTTQVNKLKAEYTSLSEEACELTQEKNELRDEKASLKSEVDNLNNQYQQRMRVLYPWVGMEPSVVMGPPPAYPYPVPVPIPSGAVPMHPQLQTYHFFHSQASGTIQNTCIPYMAYTQPCHPPTDQPSNQLNTPVAHSSSHRSNSPAQDCRSKSSTLQQPSCGVRSSDAGDIATDLELKTPGSSCQSHAEIAYNDSSSDLKTKKHCIKQINDCTLTESSSSSRCSSNGPPDVSNSAIDE

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTCTTCGTCGGGGCCTTTGCCGGCGACCGGAACCCACCACGGCAGTCAAAGGGCAGAGTGTAAATCCCAGGGTTCCACTTCTGCACGCAAGGTACAAAAGGCTGATCGTGAGAAGATGCGAAGAGATAAATTAAACGAGCAGTTCCAGGACTTGGGAAATGCACTTGATCCAGACCGTCCCAGGAACGATAAGGCAACTATACTTGGTGACACAATCCAGATGCTGAAAGATTTGACTACTCAGGTGAACAAGCTGAAAGCTGAATACACGTCACTTTCTGAAGAAGCGTGTGAGTTAACACAAGAAAAGAATGAGCTTAGAGATGAGAAAGCTTCACTGAAATCTGAGGTTGATAATTTGAATAATCAGTATCAGCAACGAATGCGGGTGCTATACCCATGGGTTGGAATGGAGCCTTCTGTTGTCATGGGGCCACCTCCTGCATATCCCTACCCAGTCCCAGTTCCAATACCCTCTGGTGCTGTACCCATGCATCCGCAGTTGCAAACATACCACTTCTTCCACAGTCAAGCTTCTGGAACCATCCAAAACACATGCATCCCTTACATGGCATATACACAACCCTGCCATCCTCCCACTGATCAGCCGTCTAACCAACTTAATACTCCAGTTGCCCATTCAAGTAGTCATAGGTCTAACTCTCCAGCTCAAGACTGTAGAAGCAAGTCATCTACATTGCAGCAACCAAGCTGTGGAGTGAGAAGCAGTGATGCTGGTGATATTGCTACTGATCTGGAATTGAAGACTCCTGGTTCTTCATGTCAATCACATGCAGAGATTGCTTACAACGATTCCTCTTCGGATTTGAAAACAAAGAAGCATTGCATAAAGCAGATTAATGATTGCACTCTCACTGAAAGCAGTAGTTCAAGCAGATGCTCTTCCAATGGTCCCCCAGATGTTTCTAATAGTGCTATTGATGAA