TFome clone pUT1803

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B204UP
Temperature (ºC)	62.0
Sequence Copy to clipboard	caccATGGACAACCTTGGGCATAGAGAAAA

3' Primer

Name	B204DN
Temperature (ºC)
Sequence Copy to clipboard	CCGGATGGTGATGTACCGGTTCGTGCC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDNLGHRENGRQRPEQYKALHTQWMIPQRQLKDHQSMNLLALMNEKDSAIRERDHALAEKKAAIAERDMAFAQRDAAMAERNAAIVERDNALAALELARTNGFNMNSGNGFHQGPPLNGTKNIHHHDHLSHVQTSPLQLANSPYDHVREMHISEAYPIATAPASVGKAKKPRKSNSQASPSKRPSGVLRKTKKATSDWKNAGTTGGAGDSARASVMKNEWKDQDLGLNQVVFDESTMPAPACSCTGELHQCYKWGSGGWQSSCCTMNMSMHPLPVMPNRRHARMGGRKMSGGAFAKLLSRLAAEGHDLSTPVDLKDHWAKHGTNRYITIR

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACAACCTTGGGCATAGAGAAAATGGCAGGCAAAGGCCTGAGCAATATAAAGCGCTTCATACTCAGTGGATGATACCTCAAAGGCAGCTAAAAGACCATCAAAGTATGAATCTCCTGGCACTGATGAATGAGAAGGACAGTGCCATCCGAGAAAGGGACCATGCTCTCGCTGAGAAGAAAGCAGCTATAGCTGAGCGAGATATGGCATTCGCCCAGCGGGATGCTGCAATGGCTGAACGGAATGCTGCAATAGTGGAGAGGGACAACGCCCTAGCTGCACTCGAACTCGCCCGTACAAATGGGTTTAATATGAATAGTGGGAATGGATTTCACCAAGGACCTCCTCTCAATGGAACAAAGAACATCCATCACCATGACCATCTGTCTCATGTCCAAACATCCCCGCTGCAGCTGGCCAATTCTCCATATGACCATGTCAGGGAGATGCACATATCAGAAGCATACCCTATCGCAACAGCTCCCGCAAGTGTTGGGAAGGCTAAGAAGCCAAGGAAGAGCAATTCTCAAGCTTCTCCATCGAAGAGGCCATCAGGTGTACTCCGAAAAACCAAGAAAGCAACCAGCGACTGGAAGAATGCGGGGACTACTGGTGGAGCAGGAGATTCAGCCCGCGCTTCTGTGATGAAGAACGAGTGGAAGGACCAGGATCTCGGCCTGAACCAGGTGGTGTTCGACGAGTCAACCATGCCCGCGCCTGCCTGCTCATGCACAGGGGAGCTCCACCAATGCTACAAATGGGGCAGTGGTGGGTGGCAGTCGTCTTGCTGCACCATGAACATGTCCATGCACCCGCTCCCGGTGATGCCCAACAGGCGCCACGCTCGCATGGGGGGAAGGAAGATGAGCGGGGGCGCGTTCGCGAAGCTGCTCAGCCGGCTGGCAGCAGAAGGTCATGACCTCTCGACTCCGGTGGACCTCAAGGACCACTGGGCCAAGCATGGCACGAACCGGTACATCACCATCCGG