TFome clone pUT1856

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B4 UP
Temperature (ºC)	62.0
Sequence Copy to clipboard	caccATGGACGACGACGACGAC

3' Primer

Name	B4 DN
Temperature (ºC)
Sequence Copy to clipboard	CTGGCTCTCTTCTTCATAATCTTC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDDDDDVSPSSSPSPPSSSALPVDGPVTVAAAPPGASMAVALPIHRTAASLYASAGGDGGGGGGREDAWSDGATSALIDAWGERFVALGRGSLRHPQWQEVAEAVSSRDGYSKAPKSDIQCKNRIDTLKKKYKVERIKPVSGWQFFDRLDFLLAPTYGNKPGNGGGGHNSNSRSQMPAAPRVGFPQRSRTPLMPAAGSGAKRRAPSPEPSVSSDSSDGFPPMPALPAVNGKRKRTDDGRSDGGGDRAQGLRELAQAIRRLGEAYERVEATKLEQEAEMERRRMDFAQELESQRVRFFLDTQMELTQAKNHASPATAAVPAVGSSRRIPRMSLVTDAGGSSNHHSRYRISHVDRHRHAPRPHYQQYHDNNNAGAAAASDGEQSDEEDYEEESQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACGACGACGACGACGTGTCGCCCTCCTCCTCCCCGTCGCCGCCCTCCTCCTCCGCGCTCCCCGTGGACGGCCCCGTCACCGTCGCCGCTGCGCCGCCGGGCGCTTCCATGGCGGTCGCGCTCCCGATCCATAGGACCGCGGCGTCCCTGTACGCTAGCGCCGGCGGCGATGGCGGCGGCGGCGGCGGGAGGGAGGACGCCTGGAGCGACGGCGCCACGTCCGCGCTCATCGACGCCTGGGGCGAGCGCTTCGTCGCGCTCGGCCGCGGCAGCCTCCGCCACCCGCAGTGGCAGGAGGTCGCCGAAGCCGTCTCCTCCCGTGACGGCTACTCCAAGGCGCCCAAGTCCGATATCCAGTGCAAAAACCGCATCGACACGCTCAAGAAGAAGTACAAGGTCGAGAGGATCAAGCCGGTCTCCGGCTGGCAGTTCTTCGACCGCCTCGATTTTCTCCTCGCGCCCACCTACGGCAACAAGCCCGGCAACGGCGGCGGCGGGCACAACTCCAACAGCCGCAGCCAGATGCCTGCGGCGCCGCGAGTGGGCTTCCCTCAACGCAGCCGCACGCCGCTCATGCCCGCAGCAGGATCCGGGGCCAAGCGGAGGGCGCCTTCGCCGGAGCCGTCGGTGTCCTCCGATTCCTCCGACGGCTTCCCGCCAATGCCGGCCCTTCCGGCGGTGAACGGGAAGAGGAAGAGGACGGACGATGGGCGCTCCGACGGAGGCGGCGACCGCGCGCAGGGGCTTCGGGAGCTGGCTCAGGCGATACGGCGCCTCGGCGAGGCGTACGAGCGCGTGGAGGCCACGAAGCTGGAACAGGAGGCCGAGATGGAGCGTCGGCGCATGGACTTCGCGCAGGAGCTGGAGTCGCAGCGCGTGCGGTTCTTCCTCGACACGCAGATGGAGCTCACGCAGGCCAAGAATCACGCGTCTCCTGCCACGGCCGCCGTCCCTGCTGTTGGCTCGTCGAGGAGGATTCCGAGGATGTCACTGGTTACCGACGCCGGTGGCAGCAGCAATCACCATAGCCGTTACCGGATCAGCCATGTTGACAGGCACCGCCATGCTCCACGCCCACATTACCAGCAGTACCATGACAACAATAACGCGGGGGCAGCGGCTGCAAGTGACGGTGAGCAGTCTGACGAGGAAGATTATGAAGAAGAGAGCCAG