TFome clone pUT1887

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B52 UP
Temperature (ºC)	60.0
Sequence Copy to clipboard	caccATGAAGGTGCTCTGCTCC

3' Primer

Name	B52 DN
Temperature (ºC)
Sequence Copy to clipboard	ACATCTCCTGCGGCGCTT

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MKVLCSACEAAEARLLCCADEAALCARCDRDVHAANRLAGKHHRLPLLPPDDVSAPNCDICQEAHAYFFCVEDRALLCRACDMAVHTANAFVSAHRRFLLTGVQVGLQPAAAAQDADPHPPAAAEPLQTPPPPDRKAAAAGDGSSPALLYSDDDIDWAAGADAGGGVSVAVTLPDWSLVHEQFGAPPVVPRHADPALARTPSSKRSPRRSVAAAFTVQGGGGLAGGLPDWPLDEFFGFSEYSAGLAFAENDTSKADSGKLGSTDGSPAGRPSSDASQDFFGQVPEFHQWSVPELPSPPTASGLHWQGGPRHGAATTTDVAAVFVPDISSPENPFWCYATAAGQAPTKRRRRC

TFome clones do not contain stop codon at 3'end.

CDS

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ATGAAGGTGCTCTGCTCCGCGTGCGAGGCGGCCGAGGCGCGCCTGCTCTGCTGCGCCGACGAGGCTGCCCTCTGCGCGCGCTGCGACCGCGACGTGCACGCCGCCAACCGCCTCGCCGGCAAGCACCACCGCCTGCCGCTCCTCCCGCCCGACGACGTCTCCGCGCCCAACTGCGACATATGCCAGGAGGCCCACGCCTACTTCTTCTGCGTGGAGGACCGCGCGCTGCTCTGCCGGGCCTGCGACATGGCCGTGCACACGGCCAACGCCTTCGTCTCCGCGCACCGCAGGTTCCTCCTCACCGGCGTCCAGGTCGGCCTGCAGCCCGCCGCCGCGGCCCAGGACGCAGACCCGCACCCGCCCGCCGCCGCGGAACCCCTCCAGACGCCCCCGCCGCCCGACAGGAAGGCGGCGGCGGCCGGCGACGGCAGCAGCCCGGCGCTGCTCTACAGCGACGACGACATCGACTGGGCGGCCGGCGCGGACGCCGGCGGCGGCGTCAGCGTCGCCGTCACCCTGCCGGACTGGTCGCTCGTCCACGAGCAGTTCGGCGCCCCGCCGGTGGTGCCCCGACACGCGGACCCGGCGCTAGCCAGAACGCCCTCTTCCAAGCGCAGTCCCCGGCGGTCGGTCGCCGCGGCGTTCACCGTCCAGGGCGGCGGCGGCCTTGCAGGGGGCTTGCCGGACTGGCCGCTCGACGAGTTCTTTGGCTTCTCCGAGTACAGCGCCGGCCTCGCCTTCGCTGAGAATGACACGTCCAAGGCCGACAGCGGGAAGCTCGGGAGCACCGACGGCTCGCCGGCCGGCCGGCCGTCGTCCGACGCGTCCCAGGACTTCTTCGGGCAGGTGCCGGAGTTCCACCAGTGGTCCGTGCCGGAGCTCCCTTCCCCGCCCACGGCCTCGGGGCTCCACTGGCAGGGCGGCCCGCGCCACGGCGCGGCCACCACCACCGACGTGGCCGCCGTGTTCGTGCCGGACATCTCGTCCCCGGAGAACCCCTTCTGGTGCTACGCCACCGCCGCTGGCCAGGCGCCGACGAAGCGCCGCAGGAGATGT