| TFome pUT3057 | |
|---|---|
| Template | BT041681 |
| Genbank Insert ID | JX428462 |
| Protein Name | ZmGBP16 |
| Species | Zea mays |
| TF Family | GeBP |
| Gene id | GRMZM2G174240 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A46G p.T16A PZE05209772762. c.A159T p.(=) PZE05209772649; c.C180T p.(=); c.C351T p.(=) All present in template FlcDNA, present in RNA-seq. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3057
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2747up |
| Temperature (ºC) | 61.4 |
| Sequence
|
caccATGTCATCCGGACGCCCGTC |
3' Primer
| Name | UT2747dn |
| Temperature (ºC) | |
| Sequence
|
AGGGCCGAGGCCTTTGATGG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MSSGRPSEPAMNAGAAPAASSPPRSKKRPSRMGDGNSSPNPSPRGSPARRSELKRRRRTSAESAALALATVAARASAAGVRSDAGDGAVERRRRPRTRAEGGDGAIRMLGAAAARVGGGRGAAQKHWSDDDEFTLLTAAVAFRDRNGRAPRLPDMAELFESIRESISPHIDQFMVYYKIKRLKSKFQHSAGPRDRRLRDLCSVLWGVGVVASSEDDSDVGERRTVPDAAAMMPVVTEVLGEYWKTNERAMAGVSLEKGLSLLGKKEGRLIETKWRQQLDQEMQSQMRRHDLAKEVCGLLTDAIKGLGP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCATCCGGACGCCCGTCTGAACCCGCCATGAACGCCGGCGCAGCGCCCGCGGCGTCGTCCCCTCCCAGATCGAAGAAGCGGCCGTCCCGCATGGGCGACGGCAACTCGAGCCCAAACCCTAGCCCCCGCGGCTCCCCCGCCCGCAGGAGCGAGCTTAAGCGCAGGCGCCGCACATCTGCCGAGTCCGCCGCGCTCGCGCTCGCCACGGTCGCCGCGCGCGCGTCCGCGGCCGGCGTCCGCTCCGACGCGGGCGACGGCGCCGTCGAGAGGCGCAGACGCCCGCGCACCCGCGCTGAAGGAGGCGATGGGGCCATCCGGATGCTCGGAGCCGCCGCCGCCCGCGTCGGTGGAGGCCGCGGCGCCGCCCAGAAACACTGGAGCGACGACGACGAGTTCACGCTCCTCACCGCCGCCGTTGCCTTCCGCGACCGCAACGGCCGGGCACCGCGCCTCCCGGACATGGCCGAGCTGTTCGAGTCCATTAGGGAGTCCATCTCCCCGCACATCGACCAGTTCATGGTGTACTACAAGATCAAGCGCCTCAAGAGCAAGTTCCAGCACTCCGCCGGCCCGCGCGACCGTCGCCTGCGCGACTTGTGCTCCGTCCTATGGGGTGTCGGCGTCGTGGCCTCTTCCGAGGACGACTCGGACGTCGGCGAGCGACGCACTGTCCCGGATGCGGCAGCCATGATGCCGGTGGTCACCGAGGTGCTCGGCGAGTACTGGAAAACGAATGAGCGAGCCATGGCAGGGGTGTCCCTTGAGAAGGGTCTGTCCCTACTGGGGAAAAAGGAGGGCAGACTCATTGAAACCAAATGGAGGCAGCAGCTTGATCAAGAGATGCAATCTCAGATGCGGCGGCATGATCTGGCTAAGGAAGTCTGCGGCTTGCTCACTGATGCCATCAAAGGCCTCGGCCCT
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