| TFome pUT3066 | |
|---|---|
| Template | BT062807 |
| Genbank Insert ID | JX428470 |
| Protein Name | ZmC3H30 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM2G123876 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3066
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2648up |
| Temperature (ºC) | 60.3 |
| Sequence
|
caccATGAATCCCCTCACGCAG |
3' Primer
| Name | UT-2648dn |
| Temperature (ºC) | |
| Sequence
|
CTCTCTGCCGTGCCTTG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MNPLTQVKRTQIINQKEALLGIGEDASWHAKFKDSAYVYVGGVPFDLTEGDLLAIFAQYGEVVDVNLVRDKGTGKSKGFAFLAYEDQRSTILAVDNLNGAKVLGRIIKVDHVSKYKKKEEEDEEELQKKREARGVCYAFQKGECNRGASCRYSHDEQRNANTGWGSKEDSGARWEHDKHSDVPKSRGMCYAFQKGECNRGASCKFLHDEQRNANSRRDSRDGGPSRSERYGDRESRSRHDDRRAEDRDRFRRDNSPERSRGERQRDDDRSDRGREAKSERRRYDVEHEDRKRSRHGRE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAATCCCCTCACGCAGGTGAAGCGGACGCAGATTATCAACCAGAAGGAGGCGCTCCTGGGCATCGGCGAGGACGCGTCGTGGCACGCCAAGTTTAAGGACTCGGCCTACGTCTACGTCGGCGGCGTCCCCTTCGACCTCACCGAGGGCGACCTCCTCGCCATCTTTGCGCAGTATGGTGAGGTTGTCGACGTGAACCTCGTGCGCGACAAGGGCACCGGAAAATCCAAGGGGTTCGCGTTCCTCGCGTACGAGGACCAGAGAAGCACGATTCTTGCTGTTGACAATTTGAATGGAGCTAAAGTTCTTGGGAGGATCATAAAGGTTGACCATGTGAGCAAGTACAAGAAGAAGGAAGAGGAAGATGAGGAGGAGCTGCAGAAGAAGAGGGAGGCCCGTGGAGTCTGCTATGCTTTCCAGAAAGGCGAGTGCAACCGCGGAGCTTCCTGCAGATATTCACATGATGAGCAGAGAAATGCAAACACCGGTTGGGGTTCTAAAGAGGATAGTGGTGCACGATGGGAGCATGACAAACACAGTGATGTACCGAAGAGTCGAGGCATGTGCTATGCTTTCCAGAAGGGAGAGTGCAACCGTGGAGCATCCTGCAAATTTTTGCATGACGAGCAGAGAAATGCAAACAGTCGTCGGGACTCTAGGGATGGTGGACCATCGAGATCAGAGCGGTATGGAGACCGAGAATCTAGGAGTAGGCATGATGATAGAAGAGCAGAAGATCGGGACAGATTCAGGCGTGACAACTCACCTGAGAGATCACGAGGTGAGAGGCAGAGGGACGATGACAGATCCGACAGAGGAAGAGAAGCAAAATCAGAAAGACGCAGATATGATGTGGAACATGAGGATCGCAAGCGGTCAAGGCACGGCAGAGAG
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