TFome clone pUT3074

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT-2553up
Temperature (ºC)	63.2
Sequence Copy to clipboard	caccATGCTGTCTTCACACCACGAGG

3' Primer

Name	UT-2553dn
Temperature (ºC)
Sequence Copy to clipboard	TGGCATGTAGAGAGCCTCCAATC

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

Copy to clipboard

MLSSHHEAMLPYAPPSLLVDRRYRQQQAGEAAPSCPRCDSPNTKFCYYNNYSLTQPRYFCKGCRRYWTRGGSLRNVPVGGGCRRNRRAKSSVRSAFGGGHRFVQGPVVRPDLVLEGMVSNSNPAHEMPAAADGSTIDLAMLYAKFLDHHPPAPEEGLGSAVTPELAVALAGPERLDEALFDTFSDLSPPAGVLAAPPVQFDPPCGHDDGFDDWSSGGPVSSAGPSSTASTATATTCIPPCADVSVHAAFGEEFNFAKDQSCLLFDSLGLPTDDVGDLSSSSWRSIVPGLSTLEDTEYDLLDSSFPDDALSLHEGMVSGTDHDCWSVVDCQGLEALYMP

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGCTGTCTTCACACCACGAGGCCATGCTGCCGTATGCGCCGCCGTCGCTGCTGGTGGACCGTCGGTACAGGCAGCAACAAGCCGGAGAGGCGGCGCCCAGCTGCCCGCGCTGCGACTCGCCCAACACCAAGTTCTGCTACTACAACAACTACAGCCTCACCCAGCCGCGCTACTTCTGCAAGGGCTGCCGCCGGTACTGGACCAGGGGCGGCTCCCTCCGCAACGTGCCCGTCGGCGGCGGGTGCCGGAGGAATCGCCGGGCCAAGTCGTCCGTGCGGTCGGCGTTCGGCGGCGGCCACCGGTTCGTCCAGGGCCCCGTCGTCCGGCCCGACCTGGTCCTGGAAGGCATGGTGTCGAACTCGAACCCAGCCCATGAGATGCCCGCCGCTGCCGACGGCTCCACCATTGATCTCGCGATGCTGTACGCCAAGTTTCTCGATCACCACCCTCCGGCGCCGGAGGAAGGCCTCGGTAGTGCCGTGACGCCGGAGCTGGCGGTGGCACTGGCTGGGCCAGAGCGGCTCGACGAGGCATTATTCGACACGTTCAGCGACCTGAGCCCACCAGCTGGCGTTCTGGCGGCGCCGCCGGTGCAGTTCGATCCGCCGTGCGGCCACGACGACGGGTTCGACGACTGGTCATCGGGCGGGCCAGTGAGCAGCGCCGGGCCCTCGAGTACGGCCAGTACTGCCACCGCAACCACATGCATCCCGCCGTGCGCCGACGTGAGCGTGCACGCTGCGTTCGGCGAGGAGTTCAACTTCGCCAAGGACCAGAGCTGCCTATTATTCGACTCGCTAGGGCTGCCCACGGACGACGTCGGCGACCTCTCTTCGTCGTCGTGGCGTTCGATCGTGCCGGGCTTGTCGACGCTGGAGGACACCGAGTATGACCTGCTGGACTCGTCGTTCCCTGACGACGCCCTCAGCCTTCACGAGGGCATGGTTAGCGGGACTGATCATGACTGCTGGAGCGTGGTGGATTGCCAAGGATTGGAGGCTCTCTACATGCCA