TFome clone pUT3077

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT2505up
Temperature (ºC)	63.7
Sequence Copy to clipboard	caccATGGCGGCGGACCAGCA

3' Primer

Name	UT2505dn
Temperature (ºC)
Sequence Copy to clipboard	GTACTTCCACACGCCATCCATCTCTAT

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MAADQQPQLQEEMNDAAGGGLRLPPGFRFHPSDFEIVSFYLTNKVLNTRFTCTAITEADLNKIEPWDLPSKAKMGEKEWYFFYQKDRKYPTGLRANRATEAGYWKATGKDKEVYNAAEGVAVLVGMKKTLVFYRGRAPRGDKTNWVMHEYRLEGSGRLPAGLASATGSAAANAAAALKASAYKDEWVVCRVFHKTTGIKKTTAAPAYQVAMAGAEMDQNQNNFPGIPFPMPMQFPMLPDFSLDPVPPYYPNAAGAGMSMLPMAAGIGGGAGGFQLNGAALFGNPMAAPQPMSFYHQMGAAGTACAGGFDVSAPESRPSSMVSQKDDQANGAEISSMMSVAGPGPATTTTIEMDGVWKY

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGGCGGACCAGCAGCCGCAGCTGCAGGAGGAGATGAACGACGCTGCCGGCGGCGGCCTCAGGCTGCCTCCAGGGTTCCGCTTCCACCCGAGCGACTTCGAGATTGTCAGCTTCTACCTCACCAACAAGGTGCTCAACACGCGCTTCACCTGCACCGCCATCACGGAGGCCGACCTAAACAAGATTGAGCCATGGGACCTCCCTAGCAAGGCGAAGATGGGCGAGAAAGAGTGGTACTTCTTCTACCAGAAGGACCGCAAGTACCCGACGGGGCTGAGGGCGAACCGGGCCACCGAGGCCGGTTACTGGAAGGCGACGGGCAAGGACAAGGAGGTCTACAACGCCGCGGAAGGGGTGGCGGTACTGGTCGGCATGAAGAAGACGCTCGTCTTCTACAGGGGCAGGGCTCCCAGGGGTGACAAGACAAACTGGGTCATGCACGAGTACAGGCTCGAAGGCAGCGGCAGGCTCCCCGCCGGCCTCGCGTCCGCAACCGGCTCAGCCGCCGCCAACGCCGCGGCGGCCTTGAAAGCTTCTGCTTATAAGGATGAGTGGGTAGTGTGTCGTGTGTTCCACAAGACCACTGGGATCAAGAAGACCACTGCTGCACCGGCGTACCAGGTGGCCATGGCCGGCGCTGAGATGGATCAGAATCAGAACAACTTCCCGGGCATCCCCTTCCCCATGCCGATGCAATTTCCCATGCTGCCAGACTTCTCCTTGGACCCGGTGCCCCCCTACTACCCCAACGCCGCTGGCGCGGGGATGTCGATGCTTCCTATGGCAGCAGGTATAGGTGGTGGCGCCGGTGGGTTCCAGCTCAACGGCGCCGCCCTGTTCGGCAATCCGATGGCCGCGCCGCAGCCCATGAGCTTCTACCACCAGATGGGCGCGGCGGGGACAGCTTGCGCTGGCGGCTTCGATGTTTCTGCGCCGGAGAGTAGGCCGTCCTCGATGGTGTCGCAGAAGGACGACCAGGCTAATGGCGCTGAGATCTCGTCGATGATGTCCGTGGCCGGCCCAGGGCCTGCGACCACCACCACCATAGAGATGGATGGCGTGTGGAAGTAC