| TFome pUT3104 | |
|---|---|
| Template | BT066632 |
| Genbank Insert ID | JX428506 |
| Protein Name | ZmARF4 |
| Species | Zea mays |
| TF Family | ARF |
| Gene id | GRMZM2G034840 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C418T p.P140S; Not in functional domain. c.T905C p.M302T; PZE0203329045 In functional domain. c.A1569T p.(=) Present in Template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3104
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2750up |
| Temperature (ºC) | 62.4 |
| Sequence
|
caccATGATGACGTCCTCGTACGAGAAGGC |
3' Primer
| Name | UT-2750dn |
| Temperature (ºC) | |
| Sequence
|
AGCCATTTGCATGCAGTCGTTCAAGA |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MMTSSYEKATSGVLRNAAALLDEMQLMGETQGAKKVINSELWHACAGPLVCLPQRGSLVYYFPQGHSEQVAATTKKIPNSRIPNYPSLPSQLLCQVHNITLHADKETDEIYAQMTLQPVHSETDVFPIPTLGAYTKSKHSSEYFCKNLTASDTSTHGGFSVPRRAAEKLFPQLDYSMQPPNQELIVRDLHDNMWTFRHIYRGQPKRHLLTTGWSLFVGAKRLKAGDSVLFIRDEKSQLLVGVRRATRQQPALSSSVLSTDSMHIGVLAAAAHAASSGGSFTVYYNPRTSPSPFVIPLARYNTATYLQPSVGMRFAMMFETEESSKRRCTGTIVGISDYEPMRWPNSKWRNLQVEWDEHGYGERPERVSLWDIETPENMVFSSPLNSKRQCLPSYGVSGLHVSSISKPQGSPFGNLQHMPGISSDIALLLLNQSAQNLGSSIACQQSSFSSIIQNAKQSYFPPTTLGASTGWNESQQQLNALGIQKGDQVSCDVQPGIDSITAPEMNVKPRVPRSTDSYSSQSISDPNSKSDPKTKTRRSKKSSSHKTISDKSEISSVPSQICDKQRHGSEPTSADFEAEQATCGNNEDSSGALTRGDFAGELQVQQVEQDGLLPPPKLESSKSPDGGKSVSSFPNQGCFSQFFEGLDWMIPPSCYQDSNGIHSVTTSDSIFNPSEGIPPSTMNADGMDAFQTSCLSECFPNSIQEFISSPDINTLTFMSPEMQHLDAQHDGSNLQSTSNSYVQMSFSEESQSASLSGLHMEAVHINSSCLQPLATGSFDAGTFSKLSNIKECQALPLQEIHNSSMGTPSCSMDAAAVEYCMDRSVKPLKPPVRTYTKVQKLGSVGRSIDVTRFRDYHELRSAIACMFGLQGKLEHPGSSDWKLVYVDYENDVLLVGDDPWEEFINCVRCIRILAPSEVQQMSENGVHVLNDCMQMA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGACGTCCTCGTACGAGAAGGCCACGTCGGGTGTCCTGAGGAACGCGGCGGCGCTGCTCGACGAAATGCAGCTCATGGGAGAAACGCAGGGTGCCAAGAAGGTGATCAACTCCGAGCTGTGGCACGCGTGCGCGGGGCCGCTGGTCTGCTTGCCGCAGCGGGGGAGCCTCGTCTACTACTTCCCCCAGGGGCACAGCGAGCAGGTTGCTGCTACCACTAAAAAGATACCCAACTCTCGCATCCCAAACTACCCGAGTCTCCCGTCGCAGCTGCTATGTCAAGTCCACAACATCACTTTGCATGCTGACAAAGAAACTGATGAGATTTATGCCCAGATGACCCTGCAACCAGTACACTCGGAAACTGATGTGTTCCCAATCCCAACGCTCGGTGCTTATACCAAAAGCAAGCACTCCAGTGAATATTTCTGCAAGAATTTGACTGCAAGTGATACAAGTACGCATGGTGGTTTCTCGGTGCCACGGAGAGCTGCAGAGAAGCTGTTTCCGCAGTTGGATTATTCAATGCAGCCTCCTAATCAGGAGCTTATCGTTCGAGATCTGCATGATAACATGTGGACATTCCGTCACATTTATCGTGGCCAGCCAAAGCGACATCTTCTAACAACTGGATGGAGTCTTTTTGTTGGTGCGAAACGGCTTAAAGCTGGTGACTCTGTCTTATTTATCAGGGATGAGAAGTCACAGCTCCTTGTGGGTGTTAGGCGGGCCACCAGACAACAACCGGCTTTGTCATCATCCGTCCTGTCTACTGACAGTATGCACATTGGTGTTCTTGCTGCTGCAGCTCATGCAGCATCAAGTGGTGGCTCGTTTACTGTTTACTACAATCCTCGGACAAGCCCGTCGCCATTTGTGATTCCTCTCGCAAGGTACAATACGGCCACATATCTGCAACCATCAGTTGGAATGAGATTCGCGATGATGTTTGAGACGGAGGAGTCAAGCAAGCGCAGATGCACAGGTACAATTGTGGGAATTAGCGACTATGAACCGATGCGATGGCCAAACTCAAAATGGCGCAACTTGCAGGTAGAATGGGATGAACATGGGTATGGAGAAAGACCTGAAAGAGTTAGTCTATGGGATATCGAAACTCCAGAGAACATGGTTTTCTCTTCCCCATTGAATTCGAAAAGGCAATGTCTTCCTAGTTATGGAGTTTCTGGCCTACATGTGTCATCAATTTCAAAGCCACAAGGAAGTCCTTTTGGTAACTTGCAGCACATGCCAGGGATCAGTTCAGACATTGCTTTGCTGCTTCTCAATCAATCTGCCCAGAACCTCGGGAGTTCAATTGCTTGTCAACAATCATCATTTTCCAGCATTATTCAAAATGCTAAGCAAAGCTATTTCCCTCCAACGACATTAGGTGCTTCCACTGGTTGGAATGAATCTCAGCAGCAGTTAAACGCCCTTGGTATTCAAAAAGGTGATCAAGTAAGCTGTGATGTTCAGCCAGGTATTGATTCAATCACTGCACCGGAGATGAATGTTAAACCAAGAGTCCCAAGAAGCACGGATTCATACTCAAGTCAGAGCATATCGGATCCAAACAGCAAGAGTGACCCTAAAACAAAAACGCGAAGGAGCAAGAAGAGCTCGTCTCACAAAACCATTTCGGATAAATCTGAGATTTCTTCAGTACCTTCTCAGATCTGTGACAAGCAAAGGCATGGTTCAGAACCAACATCGGCAGACTTTGAAGCAGAACAAGCTACCTGTGGGAACAATGAGGATTCATCTGGTGCACTCACACGTGGTGATTTTGCTGGAGAGCTACAAGTTCAGCAGGTTGAACAAGATGGTCTACTTCCACCACCGAAGTTAGAGTCATCAAAGTCACCTGATGGAGGGAAGTCCGTCAGCTCGTTCCCAAACCAAGGGTGTTTCTCACAGTTCTTTGAGGGTCTGGATTGGATGATCCCGCCTTCCTGCTACCAAGACTCCAATGGCATCCATTCAGTTACCACATCGGATAGCATCTTTAATCCGTCTGAAGGTATACCACCTTCCACAATGAACGCGGACGGCATGGACGCGTTCCAAACCTCTTGCCTTTCCGAGTGCTTTCCTAATTCCATCCAAGAATTCATCAGCAGTCCAGATATAAACACACTGACATTTATGTCGCCTGAGATGCAACATCTGGATGCCCAACACGACGGCAGCAACTTGCAGAGCACGTCCAACTCCTATGTGCAGATGAGCTTCTCGGAGGAGAGCCAGAGTGCATCCTTAAGTGGCCTACACATGGAAGCTGTCCATATCAACAGCTCCTGCCTTCAACCACTGGCGACAGGGAGCTTTGATGCAGGGACGTTCTCAAAACTATCAAACATAAAAGAATGCCAGGCCTTGCCTCTGCAGGAGATTCACAACAGCTCCATGGGAACACCTTCGTGCAGTATGGATGCGGCGGCGGTCGAGTACTGTATGGATCGAAGTGTGAAGCCACTGAAACCACCGGTCCGGACATATACAAAGGTTCAAAAGTTAGGATCAGTTGGAAGATCTATCGACGTTACACGGTTTAGAGACTACCATGAGTTGAGGTCAGCCATTGCCTGCATGTTTGGACTACAGGGGAAACTGGAACACCCTGGCAGTTCAGACTGGAAGCTTGTTTATGTCGACTACGAGAACGATGTTCTCCTCGTTGGAGACGACCCGTGGGAGGAGTTCATCAACTGTGTGAGATGCATTCGGATCCTGGCGCCTTCGGAAGTACAGCAGATGAGCGAGAATGGCGTGCATGTCTTGAACGACTGCATGCAAATGGCT
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