| TFome pUT3111 | |
|---|---|
| Template | BT055482 |
| Genbank Insert ID | JX428513 |
| Protein Name | ZmCA5P10 |
| Species | Zea mays |
| TF Family | CCAAT-HAP5 |
| Gene id | GRMZM2G440949 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3111
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2906up |
| Temperature (ºC) | 53.3 |
| Sequence
|
caccATGAGGAAGAAGCTGGACACG |
3' Primer
| Name | UT-2906dn |
| Temperature (ºC) | |
| Sequence
|
GTCTTCATTGTCATAATCCTCGTC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MRKKLDTRFPAPRIKKIMQTDEDVGKIAQAVPVLVSKALELFLQDLCDRTYDITIRKGVKTVGSSHLKQCIQTYNVYDFLREVVSKVPDIGPSDVIADDKLGKRRKAEEDGSEEELKRTRNETESHTSNGRGRGRGRGRGRRSVRGPWREVVTSPEQFKEENQSSKVASLKVEVADEVSNVTEAKQDTTPMSNARPSLRNIDLNLDPTDEEDEVTVPPEPQLSAPATSCAAATAAPGPSVPRLREGAKLKDFFGAWELADMNKMEMDPVQFALSTNHRLEEDEDYDNED
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGGAAGAAGCTGGACACGCGTTTCCCCGCTCCTCGGATTAAAAAGATCATGCAAACAGATGAAGACGTTGGCAAGATTGCTCAAGCTGTACCTGTTCTAGTGTCCAAAGCATTGGAGCTATTTCTGCAAGATTTATGTGATAGGACATATGATATTACAATTCGGAAGGGGGTCAAGACTGTGGGTTCTTCCCATTTGAAACAATGCATACAAACGTACAATGTCTATGACTTCTTGAGGGAAGTAGTCAGCAAAGTTCCAGACATTGGTCCTTCTGATGTTATTGCTGATGATAAGCTTGGCAAAAGGAGGAAAGCCGAAGAAGATGGAAGTGAGGAAGAATTGAAGAGGACAAGAAATGAGACAGAAAGCCATACAAGCAATGGCAGAGGCCGTGGGAGAGGTCGTGGGAGAGGTCGCCGTAGTGTACGTGGACCTTGGCGGGAGGTGGTCACAAGTCCTGAACAATTCAAGGAGGAGAATCAATCTAGCAAGGTAGCCAGCCTAAAGGTGGAGGTAGCAGATGAGGTCTCGAATGTAACTGAAGCAAAACAGGACACTACACCAATGAGCAATGCCAGGCCTTCCTTAAGGAACATTGACTTGAATTTAGATCCAACCGATGAAGAGGATGAGGTTACAGTGCCACCCGAACCCCAGCTGTCAGCTCCAGCAACTAGTTGCGCTGCAGCCACTGCAGCACCTGGACCATCTGTTCCACGGTTAAGGGAAGGAGCAAAACTCAAGGACTTCTTTGGTGCTTGGGAACTGGCTGACATGAACAAGATGGAGATGGACCCTGTCCAGTTTGCTCTGTCAACAAACCATAGATTGGAGGAGGACGAGGATTATGACAATGAAGAC
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