TFome clone pUT3134

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT2535up
Temperature (ºC)	61.1
Sequence Copy to clipboard	caccATGACATGGTGCAACAGCTTCAACGAC

3' Primer

Name	UT2535dn
Temperature (ºC)
Sequence Copy to clipboard	GGGACCAAAGCCAGCAGCCC

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MTWCNSFNDVRAVENNLATAAAVAAAAAKKQQQQQASSHVNLIRTCPSCGHRAQYEQVQAAATIQDLPGLPAGVKFDPTDQELLEHLEGKARPDARKLHHPLIDEFIPTIEGENGICYTHPERLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDEQGGETRWHKTGKTRPVFTGGKLKGYKKILVLYTNYGKQRKPEKTNWVMHQYHLGSDEEEKDGELVVSKVFYQTQPRQCGSGSATAKDAIPLAANAATDHHHHHHHDGGNGGGNNMLKEAGIVDFYSPAALISYNQAASNSRVAASAHLVPNFEVHTTSGAAGFGP

TFome clones do not contain stop codon at 3'end.

CDS

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ATGACATGGTGCAACAGCTTCAACGACGTGCGCGCCGTGGAGAACAACCTGGCCACCGCCGCCGCCGTGGCCGCCGCCGCCGCCAAGAAACAGCAGCAGCAGCAGGCATCCTCGCACGTCAACCTCATCAGGACCTGCCCCTCCTGCGGCCACCGCGCACAGTATGAACAGGTGCAGGCGGCGGCGACGATCCAGGACCTGCCGGGGCTGCCGGCCGGCGTGAAGTTCGATCCGACGGACCAGGAGCTGCTGGAGCACCTGGAGGGGAAGGCCAGGCCGGACGCCCGCAAGCTGCACCACCCGCTCATCGACGAGTTCATCCCCACCATCGAGGGCGAGAACGGCATCTGCTACACGCATCCGGAGAGGCTTCCTGGTGTCGGTAAGGACGGCCTCATCCGACACTTCTTCCACCGTCCGTCTAAGGCTTACACGACCGGGACGAGGAAACGACGAAAGGTCCATACCGACGAGCAGGGCGGCGAGACGCGATGGCACAAGACGGGCAAGACCCGCCCGGTGTTCACCGGTGGAAAACTCAAGGGCTACAAGAAGATCCTGGTACTCTACACCAACTACGGGAAGCAGCGGAAGCCGGAGAAGACAAACTGGGTGATGCATCAGTACCACCTAGGGTCCGATGAGGAGGAGAAGGACGGCGAGCTCGTCGTCTCCAAGGTGTTCTACCAGACGCAGCCGAGGCAGTGCGGCAGCGGTTCGGCGACGGCTAAGGACGCCATCCCGCTCGCTGCCAACGCAGCGACTGACCACCACCACCACCACCACCATGACGGTGGCAATGGCGGCGGCAACAACATGTTGAAGGAGGCAGGCATTGTAGATTTCTACAGCCCAGCAGCACTAATTAGCTACAACCAGGCAGCTTCTAACAGCAGGGTTGCCGCTTCTGCTCACCTGGTGCCTAACTTTGAGGTGCACACTACATCGGGGGCTGCTGGCTTTGGTCCC