| TFome pUT3162 | |
|---|---|
| Template | BT053836.1 |
| Genbank Insert ID | JX469913 |
| Protein Name | ZmMYB216 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G023667 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3162
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B340_F |
| Temperature (ºC) | 74.6 |
| Sequence
|
caccATGGGCGCGCCGAAGCAGAAGTG |
3' Primer
| Name | B340_R |
| Temperature (ºC) | |
| Sequence
|
AAACTCCCTTTGCGCCACTGGAACTAATG |
PCR Condition
PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo
Amino Acid Sequence
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MGAPKQKWTSEEEDALRRGVRKHGAGKWRTIQKDPQFSPILSSRSNIDLKDKWRNLSFSASGLGSSKVRVPKITGSSSSPSSSSQALLLPAANNVTEAMLPADADKKPRDGKTPPKYGAMIMEALSELNQPNGSDIDAIFDFIKQRHVVQSTFRRFLPSKLRRLADSNKIEKVDNFYRLPDSFATRTPAQIKVSDPKQKDPSKASKTIGLFAASSPALEAAMAAAVKVTDAEAKAHDAHDQMMEAERMLKMAEDTESILTIAAEIYDRCSRGEITTLVPVAQREF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCGCGCCGAAGCAGAAGTGGACGTCGGAAGAGGAGGATGCGCTCCGCCGTGGCGTTCGCAAGCACGGCGCCGGCAAATGGCGGACCATCCAGAAGGACCCCCAGTTCAGCCCGATCCTCTCCTCCCGCTCCAATATCGACCTCAAGGACAAATGGAGGAATTTAAGTTTCAGTGCAAGTGGACTGGGATCGAGTAAAGTACGGGTTCCAAAGATAACTGGATCCTCATCTTCACCATCATCTAGCTCACAGGCTCTGCTTCTCCCAGCGGCAAATAATGTTACAGAAGCTATGCTGCCTGCAGATGCTGACAAAAAGCCACGGGATGGGAAAACTCCGCCAAAGTATGGTGCCATGATTATGGAAGCTCTTTCGGAATTGAATCAGCCGAATGGTTCAGATATTGATGCAATCTTCGACTTCATAAAGCAAAGGCATGTGGTGCAATCGACCTTTAGAAGATTTCTACCCTCAAAGTTACGGCGACTTGCAGACTCAAACAAAATTGAAAAGGTCGATAACTTCTATAGGCTCCCAGATTCATTTGCAACAAGGACTCCAGCTCAAATAAAAGTTTCAGATCCAAAACAGAAGGATCCATCTAAGGCGTCAAAGACCATAGGCTTGTTTGCTGCCTCCAGCCCTGCACTAGAGGCAGCGATGGCAGCTGCTGTCAAGGTGACTGACGCCGAGGCAAAAGCACATGATGCACATGATCAGATGATGGAGGCAGAAAGGATGCTGAAGATGGCTGAGGATACCGAGTCTATCCTCACAATTGCAGCAGAGATCTATGATCGATGTTCAAGGGGGGAGATAACCACATTAGTTCCAGTGGCGCAAAGGGAGTTT
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