TFome clone pUT3217

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C269_F
Temperature (ºC)	70.7
Sequence Copy to clipboard	caccATGAGGCCAATGGCCAGCAATG

3' Primer

Name	C269_R
Temperature (ºC)
Sequence Copy to clipboard	ATGGAAGTGGTGGTGGTCGGCCCA

PCR Condition

PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo

Amino Acid Sequence

Copy to clipboard

MRPMASNGMASSLSPFFPPNFLLQMQQTPSDHDPQEHHHLPAPPLHLHHNPFLPSSQCPSLQDFRGMAPMLRKRPTMYCGADDVGVGGEEASCGATNEDEVSDDGSLQAVGPGEKKRRLNVEQVRTLEKNFELGNKLEAERKLQLARALGLQPRQVAIWFQNRRARWKTKQLEKDYDALRRQLDAVKADNDALLSHNKKLQAEILALKGREAAGGSSELINLNKETEASCSNRSEDSSEINLDISRTPPASEGPMDHPPPPPPHHAAGGGLIPFYPSVGGRHSIAAGVGMDQLLHASTPKLEQHGDGCAVAVQAAETAGSFGNLLCGVDETPPFWPWADHHHFH

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGAGGCCAATGGCCAGCAATGGCATGGCGTCCTCACTCTCGCCCTTCTTCCCTCCAAACTTCCTCCTCCAAATGCAGCAGACGCCCTCCGATCATGACCCCCAAGAACACCACCACCTTCCTGCTCCTCCCCTTCATCTTCACCACAACCCCTTCCTCCCCTCCTCTCAATGCCCCTCCCTGCAAGACTTCCGAGGTATGGCGCCAATGCTGCGGAAGCGCCCGACGATGTACTGCGGCGCGGACGACGTCGGCGTCGGAGGCGAGGAGGCGAGCTGCGGCGCGACGAACGAGGACGAGGTGTCGGACGACGGCTCCCTGCAGGCCGTCGGGCCCGGGGAGAAGAAGCGGCGGCTGAACGTGGAGCAGGTGCGGACGCTGGAGAAGAACTTCGAGCTCGGGAACAAGCTGGAGGCGGAGCGCAAGCTGCAGCTGGCGCGCGCGCTGGGCCTGCAGCCGCGGCAGGTGGCCATCTGGTTCCAGAACCGGCGCGCGCGGTGGAAGACGAAGCAGCTGGAGAAGGACTACGACGCGCTGAGGCGCCAGCTCGACGCCGTCAAGGCCGACAACGACGCCCTCCTCTCCCACAACAAGAAGCTCCAAGCCGAGATACTGGCACTGAAGGGCAGGGAGGCAGCAGGGGGGTCGTCGGAGCTGATCAACCTGAACAAGGAGACGGAGGCGTCCTGCAGCAACCGCAGCGAGGACAGCTCCGAGATCAACCTCGACATCTCGCGCACGCCGCCGGCGTCCGAGGGCCCCATGGACCATCCGCCGCCGCCGCCGCCGCACCACGCCGCCGGCGGCGGCTTGATCCCTTTCTACCCTTCCGTCGGCGGCCGACATAGTATTGCCGCCGGCGTGGGTATGGACCAACTCCTGCACGCCTCCACCCCGAAGCTGGAGCAACACGGTGACGGCTGCGCCGTCGCCGTCCAGGCCGCGGAGACCGCCGGCAGCTTCGGCAACCTCCTGTGTGGCGTCGACGAGACGCCGCCGTTCTGGCCGTGGGCCGACCACCACCACTTCCAT