TFome clone pUT3218

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C272_F
Temperature (ºC)	67.5
Sequence Copy to clipboard	caccATGGGTCATGATGAAGCTGTAGTT

3' Primer

Name	C272_R
Temperature (ºC)
Sequence Copy to clipboard	GCTAGCGGCCACGGCGT

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence

Copy to clipboard

MGHDEAVVTQNSVKAPSPPKDQPAIYPCFDWSTVQAYYGPGVLPPAFFNPGIAPGHAPPPYVWGPQTMPPAAFGKSYAAIYPHAGGFLHPLMPLVVNPLSAEPAKSGNSKDNSLNKKLKEIDGSAVSSGNSEKTSGDHSLEGSSDGNNPKVSGTPKKRSLDDRTTSETCGESGRLPALSSVRIPDTMMKPCVSTGSDFKVSGATSTEWPAKDDKESKRERRKQSNRESARRSRLRKQAETEELARRVELLTAENTSLRSEISRLTESSQKLRMENSALMEKLADGTSDQAQEASAGHQTTPTAPSSARVVKNFLWMMDGEGASRGGSGSSSRRRDHGAPMLRQLLSSGPLAADAVAAS

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGGGTCATGATGAAGCTGTAGTTACTCAGAATTCAGTAAAAGCACCATCACCACCCAAGGATCAGCCAGCTATTTATCCATGCTTTGATTGGTCGACTGTGCAGGCATATTATGGCCCAGGGGTCTTGCCACCAGCTTTCTTTAACCCTGGAATAGCGCCTGGTCATGCCCCTCCACCATATGTGTGGGGTCCGCAGACTATGCCACCGGCTGCCTTTGGAAAATCATATGCTGCAATATACCCACATGCTGGTGGTTTCCTGCATCCACTCATGCCCCTAGTGGTAAACCCATTGAGTGCGGAGCCAGCTAAGTCTGGGAACAGCAAGGATAATAGTTTAAACAAGAAACTAAAGGAAATTGATGGATCTGCTGTGTCAAGTGGCAACAGTGAGAAAACAAGTGGGGATCACAGCCTAGAAGGATCCAGCGATGGAAACAACCCAAAGGTCAGCGGAACTCCAAAGAAGAGGAGCTTAGATGATAGGACTACATCAGAAACATGTGGGGAATCAGGAAGATTGCCAGCTTTGTCCAGTGTGCGCATTCCAGACACAATGATGAAACCATGTGTGAGCACCGGTAGTGACTTCAAAGTTAGTGGTGCGACATCAACCGAATGGCCAGCTAAGGACGACAAGGAATCGAAGCGTGAAAGGAGGAAGCAGTCAAACAGGGAATCTGCCCGGCGCTCAAGGCTGAGGAAGCAGGCTGAGACTGAAGAACTGGCTAGAAGAGTAGAGCTACTGACCGCGGAGAACACGTCCCTCCGAAGCGAGATCAGCAGGCTAACGGAAAGCTCCCAGAAGCTAAGGATGGAGAACTCGGCTCTGATGGAGAAACTAGCGGACGGTACATCAGACCAAGCACAAGAAGCTTCCGCCGGCCACCAGACGACACCAACGGCGCCATCGTCGGCTCGCGTCGTCAAGAACTTCCTCTGGATGATGGATGGCGAAGGCGCGTCAAGAGGCGGCAGCGGCAGCAGCAGCCGGCGCAGGGATCACGGTGCGCCCATGCTCCGCCAGCTCCTGAGCTCGGGTCCTCTGGCGGCTGACGCCGTGGCCGCTAGC