| TFome pUT3223 | |
|---|---|
| Template | BT033858.1 |
| Genbank Insert ID | JX469961 |
| Protein Name | ZmbZIP15 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G402862 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3223
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C296_F |
| Temperature (ºC) | 68.6 |
| Sequence
|
caccATGGCGGCCAACTACCAC |
3' Primer
| Name | C296_R |
| Temperature (ºC) | |
| Sequence
|
GAATTGGGTGGATGAGGTTCTCCG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MAANYHHYQMAVHAAAAAAAWRREPDSPQLSFVSGCSSLFSISTLQDDDDDRAAAVVIAAGHALPSTPVSLAGFVGDEVDMEVQQASGDDRRSIRMMRNRESALRSRARKRAYVENLEKEVRRLVDDNLKLKKQCKELKREVAALVLPTKSSLRRTSSTQF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGCCAACTACCACCACTACCAGATGGCGGTGCACGCGGCGGCGGCGGCGGCGGCGTGGAGGAGGGAGCCGGACAGCCCGCAGCTGAGCTTCGTGAGCGGGTGCAGCTCCCTCTTCTCCATCTCCACGCTGCAGGACGACGACGACGACCGAGCCGCCGCCGTCGTCATCGCCGCCGGCCACGCGCTGCCCTCCACGCCCGTCTCGCTCGCGGGCTTCGTCGGCGACGAGGTCGACATGGAGGTGCAGCAGGCCAGCGGCGACGATCGGAGGAGCATCAGGATGATGAGGAACCGGGAGTCCGCGCTCCGCTCCAGGGCCAGGAAGAGGGCATACGTGGAGAACCTGGAGAAAGAGGTTCGCCGGCTAGTGGATGACAACTTGAAGCTCAAGAAGCAGTGCAAAGAGCTGAAACGGGAGGTAGCGGCACTGGTCCTCCCAACCAAGAGCTCACTTCGGAGAACCTCATCCACCCAATTC
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