| TFome pUT3228 | |
|---|---|
| Template | BT066907 |
| Genbank Insert ID | JX469966 |
| Protein Name | ZmC3H29 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM2G089050 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T803C p.L268P. In template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3228
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2801up |
| Temperature (ºC) | 60.4 |
| Sequence
|
caccATGGACCGGCGGCAGGAG |
3' Primer
| Name | UT2801dn |
| Temperature (ºC) | |
| Sequence
|
AAACACGTGACTAACGTGCCTCTGTG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDRRQELCRNFQRGSCKYGSHCRFVHASSQQQQQAKPNPFGFGLGSRQQQQQQSSFRAQFQQQQQQKPNPFGFGVQGSAAAQSRNAPGQAKPFQNKWVRDPSAPTKQQEAAQPPAAAHTSCTDPESCRQQIAEDFKNETPLWKLTCYAHLRSGPCDITGDISFEELRANAYEQGRQGHPLQSIVEGEKNLKNAKLTDFNNFLNTPRLSVSQTPSFPTVASFPEVKNNSAFGVSQNNGPPVFSSFSQIGVTTNMGPGSRTTPGVPTNTPFGQSTQPSHPTFPAPTFGRSDMKFGVSGPFGSQISQQSSGTFQGSNMSSFGNFSKPHASYQQSPSSSSHHRDIDRQSQDLLSGIVVPTSATNEAPVEDNKNENQDDSIWLKEKWSIGEIPLGEPPQRHVSHVF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACCGGCGGCAGGAGCTCTGCAGGAACTTCCAGCGCGGCAGCTGCAAGTACGGATCGCACTGCAGGTTTGTGCACGCCTCCTCTCAGCAGCAGCAGCAGGCGAAGCCCAACCCGTTTGGGTTCGGCTTAGGGAGTAGGCAGCAGCAACAGCAGCAGTCTTCGTTTCGTGCGCAGTTCCAGCAGCAGCAGCAGCAGAAGCCCAACCCGTTTGGATTTGGAGTGCAAGGCAGTGCGGCTGCACAGTCCAGAAACGCTCCTGGCCAGGCAAAGCCATTTCAAAATAAATGGGTAAGGGACCCCTCAGCCCCAACGAAGCAACAGGAAGCGGCACAGCCACCAGCAGCAGCCCACACCTCCTGTACAGATCCTGAGTCCTGCAGGCAACAGATTGCCGAGGATTTTAAGAATGAAACTCCACTCTGGAAGCTTACGTGCTATGCTCACCTCAGGAGCGGTCCTTGTGACATCACGGGAGATATTAGCTTTGAAGAGCTGAGAGCTAACGCGTATGAGCAAGGCAGGCAAGGGCATCCTCTGCAGTCAATAGTAGAGGGTGAAAAAAATTTGAAAAATGCAAAGCTGACGGACTTTAATAATTTTCTGAACACGCCACGTCTATCGGTATCACAAACTCCAAGCTTTCCAACTGTGGCTTCCTTCCCTGAAGTGAAAAATAACTCAGCATTTGGGGTTTCTCAAAATAATGGACCACCAGTGTTTAGTAGTTTCAGTCAAATTGGAGTAACAACTAATATGGGACCTGGGTCCAGAACTACACCAGGAGTCCCAACCAATACTCCTTTTGGTCAATCTACCCAACCAAGCCATCCAACATTTCCTGCACCTACTTTTGGTCGTTCTGATATGAAATTTGGAGTTTCAGGTCCATTTGGAAGCCAGATATCACAACAATCATCTGGGACTTTTCAAGGCTCAAACATGTCCAGTTTTGGCAATTTCTCAAAGCCTCATGCCAGCTACCAGCAGTCCCCTTCCTCCTCTAGCCATCACAGAGACATTGATAGGCAGTCACAAGATTTACTTAGTGGAATTGTGGTTCCTACAAGTGCCACAAATGAAGCTCCTGTTGAGGACAATAAAAATGAAAATCAGGATGATAGTATCTGGTTGAAGGAAAAATGGTCAATTGGAGAGATTCCGCTGGGTGAACCGCCACAGAGGCACGTTAGTCACGTGTTT
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