TFome clone pUT3231

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT-2976up
Temperature (ºC)	61.3
Sequence Copy to clipboard	caccATGGACTCAACGCTGAACCAGGTG

3' Primer

Name	UT-2976dn
Temperature (ºC)
Sequence Copy to clipboard	ATTGCTAGATGTTGGGCCATCTGAGTT

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MDSTLNQVKEESHGEGGDLMAGTVEAADGPSAAVAAAPKPMEGLHDPGPPPFLTKTYDMVDDSDTDLIVSWSATNNSFVVWDPHAFATVLLPRHFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQRHLLKNIRRRKPPAQNATNQQSIGPYLEVGHFGYDAEIDMLKRDKQLLMAEVVKLRQEQQNTKANLKAMEDRLQGTEQKQQQMMAFLARVMRNPEFLKHLVSQNEMRKELQDAISKKRRRRIDQGPEADDLGASSSLEQGSPVLFNAQDPVEFLVDGIPADLESPAFDGHGLIGPHDIDIDIDIGSTSEQQQDMPQEDLNDNFWEQLLNEGLGEENDSPVIEDDMNVLSEKMGYLNSDGPTSSN

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACTCAACGCTGAACCAGGTGAAGGAGGAGAGCCATGGGGAGGGAGGAGATTTGATGGCAGGCACGGTGGAGGCCGCGGATGGGCCGTCCGCGGCCGTCGCCGCGGCACCAAAGCCGATGGAGGGTCTGCATGACCCTGGGCCGCCGCCGTTCCTCACCAAGACATATGACATGGTCGACGACTCGGACACCGACCTGATTGTGTCTTGGAGCGCCACCAACAACAGCTTCGTGGTGTGGGATCCGCACGCCTTCGCCACGGTGCTGCTGCCCAGGCACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGGCAGCTCAACACCTATGGTTTCAGGAAGGTGGATCCTGATCGGTGGGAATTCGCGAATGAGGGCTTTTTACGGGGACAGAGACACCTCCTGAAGAACATCAGGCGTCGAAAACCTCCTGCTCAGAATGCCACAAACCAGCAGTCCATTGGGCCTTACCTTGAGGTGGGACATTTTGGATATGATGCAGAGATTGACATGTTGAAGAGAGACAAGCAGTTACTGATGGCAGAAGTGGTGAAGCTAAGGCAGGAGCAGCAGAACACAAAGGCAAATCTCAAAGCGATGGAGGATAGGCTACAAGGGACTGAACAGAAGCAGCAGCAGATGATGGCGTTCTTGGCACGTGTCATGCGGAATCCTGAATTCTTGAAGCACCTGGTTTCCCAGAATGAGATGAGGAAGGAGCTTCAAGATGCTATTTCAAAGAAAAGAAGACGGCGCATCGACCAAGGGCCTGAAGCTGATGACTTGGGGGCTAGTAGCAGCTTGGAGCAAGGTTCACCGGTCCTGTTTAATGCCCAGGACCCAGTTGAATTCCTTGTCGACGGAATCCCAGCTGACCTCGAGAGCCCAGCTTTCGATGGCCACGGCCTGATTGGGCCACATGATATTGATATTGATATTGATATTGGCAGTACCTCTGAGCAGCAGCAAGACATGCCCCAGGAGGATCTGAATGACAACTTCTGGGAGCAGTTGCTGAACGAAGGACTTGGCGAGGAGAACGATAGCCCTGTAATCGAGGATGATATGAATGTGCTGTCTGAGAAGATGGGTTATCTCAACTCAGATGGCCCAACATCTAGCAAT