| TFome pUT3235 | |
|---|---|
| Template | BT043360.1 |
| Genbank Insert ID | JX469972 |
| Protein Name | ZmWHIRLY1 |
| Species | Zea mays |
| TF Family | WHIRLY |
| Gene id | GRMZM2G155662 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3235
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-3003up |
| Temperature (ºC) | 72.3 |
| Sequence
|
caccATGCCACCGCCGGCGCCGCTCTTC |
3' Primer
| Name | UT-3003dn |
| Temperature (ºC) | |
| Sequence
|
CCTTCGCCATTCGTACTCAGACGTGGACTGCG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MPPPAPLFLSLASTPPPALMPVHHPRAPQSLTLVPPVASSRKAAAVPACPVASPRHSDYFDPRAPPPPRGDGGYGRPPNGAQDGRVFTSYSIYKGKAALSFDPRPPLFVPLDSGAYKVAKEGFVLLQFAPAVATRQYDWTRKQVFSLSVWEIGTLLTLGPTDSCEFFHDPFKGRSEEGKVRKVLKIEPTPDGNGRFFNLSVQNRLINVDESIYIPITKGEFAVIVSTFNYIIPHLMGWSTFVSSIKPEESRPYSRPQSTSEYEWRR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCCACCGCCGGCGCCGCTCTTCCTCTCGCTCGCCTCCACGCCACCGCCTGCGCTTATGCCCGTACACCACCCCAGGGCCCCGCAATCCCTAACCCTAGTCCCTCCGGTTGCCTCCTCCAGAAAGGCCGCCGCCGTTCCCGCGTGCCCCGTCGCCTCCCCGCGTCACTCCGACTACTTCGATCCGCGGGCTCCGCCGCCGCCGCGCGGGGACGGAGGGTACGGGCGCCCTCCCAACGGCGCGCAGGACGGGAGGGTCTTCACGAGCTACAGCATCTACAAGGGCAAGGCCGCGCTGTCATTCGATCCTAGGCCGCCGCTCTTCGTGCCGCTCGATTCTGGGGCGTACAAGGTGGCCAAGGAGGGCTTCGTGCTGCTGCAGTTCGCGCCCGCCGTGGCCACACGACAGTACGACTGGACGCGCAAGCAGGTGTTCTCGTTATCTGTGTGGGAGATAGGAACCCTGCTTACTCTTGGTCCAACAGATTCATGCGAGTTCTTTCATGACCCTTTCAAGGGGCGGAGCGAAGAAGGTAAAGTGCGTAAGGTTCTAAAAATTGAGCCAACACCAGATGGCAATGGTCGCTTTTTCAACCTCAGTGTCCAGAATCGGCTTATCAACGTTGATGAGAGCATTTACATCCCTATAACCAAGGGAGAGTTTGCTGTCATTGTATCAACTTTCAATTACATCATACCGCACCTCATGGGCTGGAGCACATTTGTAAGTTCAATCAAACCCGAGGAGTCACGGCCATACAGCAGGCCGCAGTCCACGTCTGAGTACGAATGGCGAAGG
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