| TFome pUT3236 | |
|---|---|
| Template | BT043138 |
| Genbank Insert ID | JX469973 |
| Protein Name | ZmABI3 |
| Species | Zea mays |
| TF Family | ABI3-VP1 |
| Gene id | GRMZM2G149940 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G810T p.E270T. In template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3236
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2968up |
| Temperature (ºC) | 61.4 |
| Sequence
|
caccATGGCCAACGCCAATGGCTC |
3' Primer
| Name | UT-2968dn |
| Temperature (ºC) | |
| Sequence
|
CGTGATGATCTTCTTATTGATCGAAGGAC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MANANGSSTGAGHSDLVRAISHEQHQAFMASVPRAAPGGVNVHHQQHFHQYPAGLIPAPVALPVHAPVSSQTSPYSAQIAVPPPPPPLIASPDHRLHSLPPTGCYQLDYSPYGNAAAPSQQHTSAIRGFADWGTHSNALMSLAHATSFGNNGSSNINNNGLLHQNLSPYTTHTWTTTYVQRPYNTAVYAPATMNMLQTPPFHSNSHEKESGAVFSNSFNMAPSVTPTSPFQLMSPSSTNYTSTQIFEETNNLEDTSRVFGGGDNESNNSDEPDPKPAVEMEDLNQGNDHTSNKTANCQDYRMVLRKDLTNSDVGNIGRIVLPKKDAEPNLPILEDKDGLILEMDDFELPVVWNFKYRYWPNNKSRMYILESTGEFVKRHGLQAKDILIIYRNKKSGRYVARAVKAEDIAPPECECVEAGNPREECGFSVSPSINKKIIT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCAACGCCAATGGCTCGTCCACCGGTGCAGGCCATAGCGACCTCGTCAGGGCCATTTCGCACGAGCAGCACCAGGCGTTCATGGCCTCCGTGCCCCGGGCCGCACCGGGCGGGGTGAATGTGCACCACCAGCAGCACTTCCACCAATACCCCGCCGGCTTGATCCCAGCGCCGGTGGCACTGCCGGTTCACGCACCGGTGTCGTCACAGACGTCTCCGTACTCCGCCCAGATCGCGGTGCCGCCGCCGCCGCCACCACTCATCGCATCTCCAGACCACCGCCTGCACTCCCTGCCGCCTACCGGATGCTACCAGCTGGACTATTCTCCATATGGCAACGCTGCTGCGCCTTCGCAGCAGCACACATCAGCAATAAGGGGTTTTGCTGACTGGGGAACGCACAGCAATGCGCTCATGTCCTTGGCTCATGCCACTAGTTTTGGTAACAACGGGAGCAGCAATATCAACAACAATGGATTATTACATCAAAACTTATCCCCGTACACCACGCATACATGGACTACTACTTACGTGCAGAGGCCTTATAACACTGCTGTTTATGCTCCTGCAACAATGAATATGCTCCAAACTCCTCCATTTCATAGCAACAGCCATGAAAAAGAGTCAGGTGCAGTTTTTTCAAATAGCTTTAACATGGCCCCATCAGTGACACCTACCTCCCCTTTTCAACTTATGTCTCCAAGCTCAACCAACTACACTTCCACTCAAATCTTCGAGGAAACCAACAATTTAGAGGACACTTCAAGAGTCTTTGGAGGTGGGGATAACGAAAGCAACAACAGTGATGAACCGGATCCCAAGCCGGCTGTGGAGATGGAAGACCTAAACCAAGGCAATGATCATACTTCGAATAAAACAGCTAACTGTCAAGATTACCGTATGGTCCTGCGCAAGGACTTGACAAACAGTGATGTTGGAAACATTGGAAGAATCGTGCTGCCAAAGAAAGATGCAGAGCCTAACCTTCCAATCTTGGAAGATAAGGATGGCCTGATACTGGAAATGGACGATTTTGAACTCCCGGTTGTATGGAATTTCAAGTACAGGTACTGGCCTAATAACAAGAGTAGGATGTATATATTGGAAAGTACTGGTGAATTTGTTAAGAGGCATGGGCTTCAAGCTAAGGACATCCTCATCATTTACAGGAACAAGAAATCTGGCAGATATGTTGCCCGTGCGGTGAAGGCTGAGGACATAGCGCCGCCAGAATGCGAGTGCGTCGAGGCAGGAAACCCTAGAGAAGAGTGCGGCTTCTCCGTCAGTCCTTCGATCAATAAGAAGATCATCACG
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