| TFome pUT3341 | |
|---|---|
| Template | BT039053 |
| Genbank Insert ID | JX469981 |
| Protein Name | ZmGLK38 |
| Species | Zea mays |
| TF Family | G2-like |
| Gene id | GRMZM2G113742 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3341
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2914up |
| Temperature (ºC) | 60.7 |
| Sequence
|
caccATGTTCCCCAGCCTGATCCACC |
3' Primer
| Name | UT2914dn |
| Temperature (ºC) | |
| Sequence
|
ACGTGATTTGCGCTTGAATTGTTC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MFPSLIHHASHGRPDVAVAEDAPRSGCGLGLPGHGGGPSVVLTADPKPRLRWTADLHDRFVDAVAQLGGPDKATPKAIMRTMGVKGLTLFHLKSHLQKYRLGRQSGKELTEQSKDASYLMEAQSGTTLSPRGSTPDVKESQEVKEALRAQMEVQRRLHEQVEVQKHMQIRMEANQKYIDTILDKAFKIVSEQLSGFSISDQDLPILTSARAMLSPADHLSSSVFPQLSVSSVSLHNPGPGGGKGLPHVADSHVFSQRPPEQFKRKSR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTTCCCCAGCCTGATCCACCACGCTAGCCACGGCCGACCCGACGTCGCCGTCGCCGAGGACGCACCACGCAGTGGCTGCGGGTTAGGGCTGCCCGGGCACGGCGGCGGGCCCAGCGTGGTGCTCACGGCCGATCCCAAGCCTAGGCTCCGCTGGACGGCCGACCTCCACGACCGCTTCGTCGATGCCGTCGCCCAGCTCGGCGGGCCTGATAAAGCAACACCGAAAGCTATCATGAGGACAATGGGAGTGAAGGGCCTCACCCTTTTTCACTTGAAAAGCCATCTTCAGAAATACAGATTGGGAAGACAGTCTGGAAAAGAGTTAACGGAGCAATCAAAAGATGCTTCTTACCTTATGGAAGCTCAAAGTGGAACAACTTTGTCTCCTAGAGGTTCTACTCCAGATGTGAAAGAGAGTCAAGAAGTTAAAGAAGCACTCAGAGCACAAATGGAAGTGCAACGAAGATTGCACGAACAAGTGGAGGTCCAGAAGCATATGCAGATCCGAATGGAGGCGAACCAGAAGTACATCGACACGATACTAGATAAGGCGTTCAAGATAGTATCTGAGCAACTGAGTGGCTTCAGCATATCTGACCAAGACCTACCGATTCTCACCTCTGCTAGGGCCATGCTTAGCCCTGCAGATCACTTGAGTTCGTCTGTCTTCCCCCAGCTCTCCGTCAGCTCCGTTAGCCTTCACAATCCTGGTCCTGGAGGAGGGAAAGGCCTTCCGCATGTTGCCGACAGCCACGTGTTCTCCCAGAGGCCACCTGAACAATTCAAGCGCAAATCACGT
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