| TFome pUT3351 | |
|---|---|
| Template | BT060994 |
| Genbank Insert ID | JX469991 |
| Protein Name | ZmABI1 |
| Species | Zea mays |
| TF Family | ABI3-VP1 |
| Gene id | GRMZM2G133398 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3351
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2785up |
| Temperature (ºC) | 69.9 |
| Sequence
|
caccATGGAAGCCTCCGCCGGCTCGTC |
3' Primer
| Name | UT-2785dn |
| Temperature (ºC) | |
| Sequence
|
GATGCTCACCGCCATCTGGCTCATG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MEASAGSSPPHSQENPPEHGGDMGGAPAEEIGGEAADDFMFAEDTFPSLPDFPCLSSPSSSTFSSNSSSNSSSAYTNTAGRAGGEPSEPASAGEGFDALDDIDQLLDFASLSMPWDSEPFPGVSMMLENAMSAPPQPVGDGMSEEKAVPEGTTGGEEACMDASEGEELPRFFMEWLTSNRENISAEDLRGIRLRRSTIEAAAARLGGGRQGTMQLLKLILTWVQNHHLQRKRPRDVMEEEAGLHVQLPSPVANPPGYEFPAGGQDMAAGGGTSWMPHQQAFTPPAAYGGDAVYPSAAGQQYSFHQGPSTSSVVVNSQPFSPPPVGDMHGANMAWPQQYVPFPPPGASTGSYPMPQPFSPGFGGQYAGAGAGHLSVAPQRMAGVEASATKEARKKRMARQRRLSCLQQQRSQQLSLGQIQASVHLQEPSPRSTHSGPVTPSAGGWGFWSPSSQQQVQNPLSKSNSSRAPPPSLEAAAVAPQTKPAPAGARQDDIHHRLAAASDKRQGAKADKNLRFLLQKVLKQSDVGSLGRIVLPKEAEVHLPELKTRDGISIPMEDIGTSRVWNMRYRFWPNNKSRMYLLENTGEFVRSNELQEGDFIVIYSDVKSGKYLIRGVKVRPPPAQEQGSGSSGGGKHRPLCPAGPGRAAAAGAPEDAVVDGVSGACKGRSPEGVRRVRQQGAGAMSQMAVSI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAAGCCTCCGCCGGCTCGTCGCCACCGCACTCCCAAGAGAACCCGCCGGAGCACGGTGGCGACATGGGAGGGGCCCCCGCGGAGGAGATCGGAGGGGAGGCGGCGGATGATTTCATGTTCGCTGAAGACACGTTCCCCTCCCTCCCGGACTTCCCTTGCCTTTCGTCGCCGTCCAGCTCCACCTTCTCGTCCAACTCCTCGTCAAACTCCTCCAGCGCCTACACCAACACGGCAGGAAGAGCCGGCGGCGAGCCCTCCGAGCCTGCTTCGGCCGGAGAAGGGTTTGATGCGCTCGATGACATCGACCAGCTCCTCGACTTCGCGTCGCTTTCCATGCCGTGGGACTCCGAGCCGTTCCCGGGGGTTAGCATGATGCTAGAGAACGCCATGTCGGCGCCGCCGCAGCCGGTGGGCGACGGCATGAGTGAAGAGAAAGCCGTGCCGGAAGGGACCACAGGGGGAGAGGAGGCCTGCATGGATGCGTCGGAGGGGGAGGAGCTGCCGCGGTTCTTCATGGAGTGGCTCACGAGCAACCGCGAAAACATCTCGGCCGAGGATCTCCGCGGGATCCGCCTCCGCCGCTCCACCATCGAGGCCGCCGCCGCCCGGCTCGGCGGCGGGCGCCAGGGCACCATGCAGCTGCTCAAGCTCATCCTCACCTGGGTGCAGAACCACCACCTCCAGAGGAAGCGCCCGCGCGACGTGATGGAGGAGGAGGCGGGCCTGCACGTCCAGCTCCCCAGCCCGGTCGCCAACCCACCAGGATACGAGTTCCCCGCCGGCGGACAGGACATGGCCGCGGGCGGCGGCACATCTTGGATGCCCCACCAGCAGGCATTCACGCCGCCTGCTGCGTACGGCGGCGACGCGGTGTACCCGAGCGCGGCAGGCCAACAGTACTCTTTCCACCAGGGCCCCAGCACGAGCAGCGTGGTCGTGAACAGCCAACCGTTCTCCCCGCCGCCTGTGGGCGACATGCACGGCGCGAACATGGCCTGGCCGCAGCAGTACGTGCCGTTCCCACCGCCTGGGGCTTCCACGGGCTCTTACCCTATGCCGCAGCCGTTCTCCCCCGGATTCGGCGGGCAGTACGCCGGCGCCGGCGCTGGCCACCTCTCAGTGGCCCCCCAGCGCATGGCAGGCGTGGAGGCCTCGGCGACCAAGGAGGCCCGCAAGAAGCGCATGGCGAGACAGCGGCGCCTGTCCTGCCTGCAGCAGCAGCGCAGCCAGCAGCTGAGCCTGGGCCAGATCCAGGCCTCCGTCCACCTGCAGGAGCCGTCCCCTCGGTCCACGCACTCCGGCCCGGTCACGCCGTCAGCAGGCGGCTGGGGATTCTGGTCGCCGAGCAGCCAGCAGCAGGTCCAGAACCCGCTCTCCAAGTCCAATTCATCAAGGGCGCCGCCTCCCTCGCTGGAAGCGGCGGCGGTGGCGCCACAGACAAAGCCCGCGCCTGCTGGTGCTCGGCAGGACGACATTCACCACCGCCTCGCAGCGGCTTCAGATAAGCGGCAGGGCGCCAAGGCGGACAAGAACCTGCGGTTCCTGCTGCAGAAGGTGCTGAAGCAGAGCGACGTCGGGAGCCTCGGCCGCATCGTGCTCCCCAAAGAAGCGGAGGTTCACCTGCCGGAGCTGAAGACGAGGGATGGCATCTCCATCCCCATGGAGGACATCGGAACGTCGCGCGTGTGGAACATGCGGTACAGGTTTTGGCCCAACAACAAGAGCAGAATGTATCTGCTGGAGAACACAGGGGAATTTGTTCGTTCCAACGAGCTTCAGGAGGGGGATTTCATAGTGATCTACTCCGATGTCAAGTCGGGCAAATATCTGATACGGGGCGTGAAGGTAAGGCCCCCGCCGGCGCAAGAGCAAGGCAGTGGTTCCAGCGGGGGAGGCAAGCACAGGCCCCTCTGTCCAGCAGGTCCAGGGAGAGCCGCAGCCGCCGGTGCTCCTGAAGACGCCGTCGTCGACGGGGTCAGCGGCGCCTGCAAGGGGAGGTCTCCGGAAGGCGTGCGGCGGGTTCGGCAGCAGGGAGCCGGCGCCATGAGCCAGATGGCGGTGAGCATC
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