| TFome pUT3353 | |
|---|---|
| Template | EC878985 |
| Genbank Insert ID | JX469993 |
| Protein Name | ZmC3H2 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM2G306357 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A1426G, pK475R. Not present in template FlcDNA. Not present in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3353
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2663up |
| Temperature (ºC) | 66.7 |
| Sequence
|
caccATGGAGGATGCCCTCGCGC |
3' Primer
| Name | UT-2663dn |
| Temperature (ºC) | |
| Sequence
|
ACTGCCAGCACCAAACAAGAACTCC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MEDALAPPSVAPTPSPLAAAAVLTRRRSHLDSASFRTLSRLFSHCLHINPSPCGISPPVPEPVAADFVGGDPDDSPERPNGADFDRLKDVQIEAADAGGPSLHGTVHQPAAANSTLDLVEAEAEALQLEGADAVGVVESTSCMTRAEAGESAVLVVESTSCKTTAEVEESAVGAGLAMVGDVSLKSVESLLETKVDESVEVAVGDDEGCLLFEAMLTNFTGLIDDVDAGAIPTQRCAVSGGDLQNSKASEDLKQLGDRIEEGEPLRNCDNGQNDGGGFEEGEIEGEFQDLGSEESGNSEHGDEDGEDEKLEDNSISRGSGPNKSCDYGTQFGNLQSIPETGNDHLILNRDAIVRGDAQISVTRAQAVTYDEVVDWNETPLPDNETVNPGKKRKRILTEERKAKKTKNKRKNRALQRIADGVKRPKLTPVTKPKKPCHFYDHGKCRQGNNCKFSHDFTPSTKSKPCTHFACGSCLRGEECPYDHELSKYECHNYKNNGTCARGDNCKFSHVMPTTEGTPTQDAKTSDASLAYEKTNTREHTRSQKNATVHNVAPSNSILRNLAGVSSKSQNVSNHIPKGVQFLSIGKGQSNLDSLHLDALFMEKQRNANGTQHNLGGHEAERQKIVKQNSQKPNLLLDDKNSSEGATRHPCSDPQKATLPVNSIATPGSIHTQHEVSEASRILQEFLFGAGS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGATGCCCTCGCGCCGCCGTCTGTGGCCCCGACTCCCTCCCCCCTCGCCGCCGCTGCCGTCCTCACCCGTCGTCGGTCACACCTCGACAGTGCCTCCTTCCGCACGCTCTCCCGCCTCTTCTCTCACTGTCTCCACATCAACCCGTCGCCCTGTGGCATCTCGCCGCCCGTGCCCGAGCCCGTCGCCGCGGACTTCGTCGGTGGGGACCCCGACGATTCCCCAGAAAGGCCTAATGGCGCTGATTTCGATCGGCTAAAGGATGTGCAAATCGAGGCGGCGGATGCGGGAGGCCCTTCGCTTCACGGGACTGTCCATCAACCGGCCGCGGCGAACTCTACCCTCGACCTGGTTGAGGCTGAGGCTGAGGCTCTGCAGCTCGAGGGTGCGGACGCGGTAGGGGTCGTAGAAAGCACCTCGTGTATGACCAGAGCCGAGGCTGGGGAATCAGCGGTACTTGTGGTAGAAAGCACCTCATGTAAGACCACTGCTGAGGTGGAGGAGTCAGCGGTTGGAGCTGGACTGGCGATGGTGGGTGACGTATCTTTGAAGTCGGTGGAGTCTCTCTTGGAGACGAAGGTTGACGAATCGGTAGAGGTAGCAGTGGGTGATGATGAAGGCTGTCTTTTGTTTGAAGCGATGCTGACAAACTTCACAGGGTTGATTGATGATGTTGATGCTGGAGCAATACCAACACAGAGGTGTGCAGTTTCTGGAGGTGACCTGCAGAATAGCAAGGCATCTGAGGATTTGAAACAACTGGGTGATAGGATTGAAGAAGGAGAACCTCTTAGAAACTGTGATAATGGGCAGAATGATGGTGGTGGTTTCGAGGAAGGAGAGATTGAGGGCGAGTTTCAGGATTTGGGTTCAGAAGAATCTGGAAATTCCGAACATGGAGACGAAGATGGTGAAGATGAGAAATTGGAAGACAATTCCATTAGCAGAGGCTCAGGGCCAAATAAATCTTGTGATTATGGCACACAATTCGGGAATTTGCAATCAATTCCTGAAACAGGAAATGATCATCTTATACTGAACAGGGATGCTATTGTCAGAGGTGATGCACAAATATCTGTTACCAGGGCTCAAGCCGTCACTTATGATGAAGTTGTGGATTGGAATGAGACTCCGCTGCCTGACAATGAGACTGTCAACCCTGGGAAGAAGAGAAAACGCATTCTGACAGAAGAGAGAAAAGCTAAAAAAACAAAAAATAAAAGAAAGAACCGGGCACTGCAACGGATAGCGGATGGAGTCAAAAGACCAAAACTTACTCCTGTTACAAAGCCAAAAAAACCTTGCCACTTCTATGACCATGGGAAGTGCCGCCAAGGAAATAATTGCAAGTTTTCCCATGATTTTACACCTTCAACAAAATCTAAGCCCTGCACACATTTTGCATGTGGTTCTTGTTTGAGAGGAGAAGAGTGTCCATATGACCATGAGTTGTCAAAGTACGAATGCCATAACTATAAAAACAATGGAACATGTGCCAGAGGTGATAATTGCAAATTCTCTCATGTGATGCCAACTACAGAAGGTACTCCCACACAAGATGCAAAGACTTCTGATGCTTCACTGGCATATGAGAAAACAAACACCAGAGAGCATACAAGAAGCCAGAAGAACGCAACAGTTCACAATGTTGCTCCTAGTAATTCTATCCTAAGAAACCTGGCTGGCGTCTCCAGCAAATCCCAGAATGTTTCTAACCATATTCCAAAGGGCGTGCAGTTTCTTTCCATTGGTAAAGGTCAATCCAATCTGGACAGCCTGCACCTGGATGCTTTGTTCATGGAGAAGCAAAGGAATGCAAATGGTACTCAACATAACCTTGGAGGACATGAAGCAGAAAGGCAAAAAATTGTGAAGCAAAATAGCCAGAAACCGAATCTATTGCTAGACGATAAGAATTCATCAGAAGGAGCTACTAGGCATCCATGTTCAGACCCCCAAAAAGCTACTTTACCTGTTAATTCTATTGCAACACCTGGATCAATACATACTCAGCATGAAGTCTCAGAGGCTTCTAGGATTTTGCAGGAGTTCTTGTTTGGTGCTGGCAGT
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