| TFome pUT3355 | |
|---|---|
| Template | BT088318 |
| Genbank Insert ID | KJ726816 |
| Protein Name | ZmARF18 |
| Species | Zea mays |
| TF Family | ARF |
| Gene id | GRMZM2G035405 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3355
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C478F |
| Temperature (ºC) | 67.9 |
| Sequence
|
caccATGTGGCTTCGAGATACCGC |
3' Primer
| Name | C478R |
| Temperature (ºC) | |
| Sequence
|
TCCCAACTCGACCGAACCGA |
PCR Condition
Amino Acid Sequence
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MAKDLHGNEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNDNNQLLLGIRRANRPQTVMPSSVLSSDSMHIGLLAAAAHAASTNSRFTIFYNPRASPSEFVIPLVKYVKAVYHTRISVGMRFRMLFETEESSVRRYMGTITGICDLDSVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYPSPFPLRLKRPWPTGLPSLHGGKDDDLANSLMWLRDTANPGFQSLNFGGLGMSPWMQPRLDATLLGLQPDMYQAMATAAFQDATKQASPTMLQFQQPQNIAGRASPLLSSQILQQAHHQFQQQPYLQNISESTIQAQGQSEFLKQQIQRSQSFNEQKPQMQHQQESQQQQQPQCLPVPQHQQMQQQNMTNYQSISNALSPFSQLSPVSQSSPMALQTILPFSQAQSFTDTNVGSLSPSNGNTMQNTLRPFSSEAVSHLSMPRPTAIPVADPWSSKRVAVESLLPSRPQVSSQMEQLDPAPASIPHSSALAPLPGRGCLVDQDVNSDPQNHLLFGVSIDSQSLLMQGGIPGLQNGNDSAAIPYSTSNFLSPSQNDFPLDHTLNSSGCLDDSGYVPPCSDNSDKVNRPPATFVKVYKSGTYGRSLDITRFSSYHELRRELGRLFGLEGQLEDPLRSGWQLVFVDREEDVLLVGDDPWQEFVSTVSCIKILSPQEVQQMGKQGLELLSSAPARRLGSSCDDYVSRQESRSLSTGIASVGSVELG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCAAAAGATCTTCATGGCAATGAGTGGAAATTCCGTCACATCTTTCGTGGCCAGCCAAAGCGGCATCTTCTAACTACAGGTTGGAGTGTCTTTGTAAGTGCAAAGAGACTCGTTGCTGGCGACTCTGTCCTTTTTATCTGGAATGACAATAACCAGCTTCTGCTGGGAATTCGTCGTGCAAATCGGCCACAAACTGTCATGCCATCTTCAGTCTTATCAAGCGATAGCATGCATATCGGTCTTCTTGCTGCAGCTGCTCATGCTGCTTCGACAAATAGCCGATTTACAATTTTTTACAACCCAAGGGCAAGCCCTTCAGAGTTTGTCATACCACTCGTAAAGTATGTTAAGGCTGTGTATCATACCCGCATATCTGTGGGGATGCGATTCAGGATGCTTTTTGAGACAGAAGAGTCTAGCGTTAGGAGATACATGGGGACAATTACCGGAATCTGTGATCTTGATTCTGTTCGGTGGCCAAATTCACACTGGCGTTCTGTTAAGGTTGGCTGGGATGAGTCAACTGCTGGTGAGCGACAACCAAGGGTGTCACTATGGGAGATTGAACCCCTGACAACTTTCCCAATGTATCCATCTCCTTTTCCGCTCAGGCTCAAGCGTCCATGGCCAACAGGCTTGCCTTCTCTGCATGGTGGGAAGGATGATGACCTGGCTAACTCTCTCATGTGGCTTCGAGATACCGCAAATCCTGGTTTTCAGTCGTTAAATTTTGGTGGACTTGGTATGAGCCCTTGGATGCAGCCAAGGCTGGATGCTACCTTACTTGGTCTGCAACCTGACATGTATCAGGCGATGGCCACAGCTGCTTTTCAGGATGCAACAAAGCAGGCATCGCCCACAATGCTGCAGTTCCAGCAGCCCCAGAACATAGCTGGCCGGGCTTCCCCGCTTCTTTCAAGTCAGATTTTGCAGCAAGCACATCATCAATTTCAGCAGCAACCGTACCTTCAAAACATCTCTGAGAGCACAATCCAAGCACAGGGTCAGTCTGAGTTCCTCAAGCAGCAGATCCAACGCAGCCAGTCATTCAATGAGCAGAAGCCCCAGATGCAACACCAGCAAGAATCACAGCAGCAGCAGCAACCACAGTGTCTGCCAGTCCCCCAACATCAGCAAATGCAACAACAGAATATGACCAACTACCAGTCTATATCTAATGCATTATCACCGTTTTCTCAGCTGTCGCCAGTTTCTCAGTCTTCACCTATGGCGCTGCAGACTATATTACCGTTCTCGCAGGCACAGAGCTTCACAGACACGAATGTAGGTTCATTGTCTCCATCCAATGGTAACACCATGCAAAACACACTGAGGCCGTTCTCATCAGAAGCAGTTTCTCACCTCAGTATGCCGAGGCCCACTGCGATACCTGTCGCAGACCCATGGTCGTCGAAGCGTGTCGCGGTGGAGTCTCTGCTTCCTTCTCGGCCTCAGGTTTCATCCCAGATGGAACAATTGGACCCTGCACCAGCTAGTATACCTCATAGCTCTGCGTTGGCACCACTTCCCGGAAGAGGGTGCTTGGTTGACCAAGATGTGAACTCTGATCCTCAAAATCATCTCTTGTTCGGTGTTAGTATAGACTCGCAGTCACTGCTAATGCAAGGGGGCATTCCTGGTCTCCAAAACGGGAATGATTCAGCTGCTATACCTTATTCCACTTCCAATTTCCTCAGCCCTTCTCAGAATGACTTTCCTCTGGACCATACACTAAATTCTTCAGGCTGCTTAGATGATTCTGGGTACGTCCCGCCATGTTCAGATAATTCTGACAAAGTGAACCGACCACCAGCGACCTTCGTGAAGGTTTACAAATCTGGAACCTACGGGAGGTCGCTTGATATCACTAGGTTTAGCAGCTATCACGAGCTCCGTAGGGAACTGGGGCGCCTATTTGGCCTTGAGGGCCAGTTGGAAGACCCATTGAGATCAGGCTGGCAGCTTGTATTCGTCGACCGTGAGGAGGACGTCCTTCTCGTGGGCGACGACCCTTGGCAGGAATTCGTGAGCACGGTGTCCTGCATAAAGATCCTCTCCCCGCAGGAGGTGCAGCAGATGGGGAAGCAGGGCCTGGAGCTCCTGAGCTCGGCCCCCGCGCGGAGGCTCGGTAGCAGCTGTGACGACTACGTCAGCAGGCAGGAGTCGAGAAGCCTGAGCACCGGGATCGCGTCCGTCGGTTCGGTCGAGTTGGGA
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