| TFome pUT3358 | |
|---|---|
| Template | DR968328 |
| Genbank Insert ID | KJ726819 |
| Protein Name | ZmMYB182 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G123308 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.805del69. EST does not cover alternate splice site.Splice site used by T01. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3358
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2792up |
| Temperature (ºC) | 61.5 |
| Sequence
|
caccATGGAGCTCTTCCCCTCCCAGC |
3' Primer
| Name | UT2792dn |
| Temperature (ºC) | |
| Sequence
|
GTGCCTGATGCGTGGGCCTAG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MELFPSQPDLSLQIGLPASATPHDHHHGAALSARFLAAAAGGGGNGIPGNPAVAMAPSLQLPMPMPLPMPMPMPPNAAAAAGAAGGLYYHPDAAAMLRPIRGVPLYQHPHTHAVPPTFPPHAAGAAGPCFCEPCHVAAGAWRRAGCGVGTRVVAGFPTAAKRAARAPRMRWTSTLHARFVHAVELLGGHERATPKSVLELMDVKDLTLAHVKSHLQMYRTVKNTERPAASSDLADGFENGSAGEICDDNSLDLHGAGRPESGAARHGREDWTGFPSESNTGRMSMHSRSLKDQTMQSKSLEILSDMNSSCVSETTSCASELNLEFTLGPRIRH
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGCTCTTCCCCTCCCAGCCGGACCTCTCCCTCCAGATCGGCCTCCCCGCCAGCGCCACGCCGCACGACCACCACCACGGCGCCGCCCTCAGCGCCAGGTTCCTCGCGGCAGCCGCAGGCGGCGGTGGCAACGGGATCCCTGGCAACCCGGCGGTGGCCATGGCGCCGTCGCTGCAGCTGCCCATGCCGATGCCGCTGCCAATGCCGATGCCGATGCCCCCCAACGCCGCCGCCGCCGCGGGGGCCGCCGGCGGCCTTTACTACCACCCCGACGCCGCCGCCATGCTGCGGCCCATACGCGGGGTGCCGCTGTACCAGCACCCGCACACGCACGCGGTGCCGCCGACGTTCCCGCCGCACGCGGCGGGGGCGGCGGGCCCGTGCTTCTGCGAGCCTTGCCACGTCGCCGCGGGCGCCTGGCGCCGCGCCGGGTGCGGCGTCGGGACGCGCGTCGTCGCCGGGTTCCCCACGGCGGCCAAGCGCGCCGCCAGGGCGCCGCGCATGCGCTGGACGTCCACGCTCCACGCGCGCTTCGTGCACGCCGTCGAGCTCCTCGGCGGCCACGAAAGGGCAACACCCAAGTCAGTTCTTGAGCTCATGGACGTGAAGGATCTCACCCTGGCTCATGTCAAGTCTCACTTGCAGATGTACAGGACCGTGAAGAACACCGAAAGGCCGGCAGCTTCGTCAGATCTAGCTGATGGGTTCGAGAACGGGTCGGCAGGCGAGATCTGCGACGACAACTCGCTCGATCTGCACGGCGCCGGCAGGCCAGAGTCGGGGGCGGCGCGGCATGGAAGGGAAGACTGGACCGGCTTTCCCAGCGAGTCCAACACCGGGCGCATGAGCATGCATTCTCGATCTCTCAAGGATCAGACGATGCAGTCCAAGAGCCTGGAGATCCTCTCGGACATGAACTCCTCCTGCGTGTCGGAGACGACGTCGTGTGCCAGCGAGTTGAACCTGGAGTTCACCCTAGGCCCACGCATCAGGCAC
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