| TFome pUT3374 | |
|---|---|
| Template | BT054447 |
| Genbank Insert ID | KJ726834 |
| Protein Name | ZmC3H32 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM5G842019 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3374
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2688up |
| Temperature (ºC) | 62.5 |
| Sequence
|
caccATGGGCGACCTTGCTGATC |
3' Primer
| Name | UT-2688dn |
| Temperature (ºC) | |
| Sequence
|
AAAGCTCAGGTCAGGCTTGTTAATC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MGDLADLAGAGEAARSHLAAGGQRDRLAALLELAAADDVAGFREALAGGGGEGDTAELADGVGMWYGRSKAYEPRTPLMVAATYGSVEVVSLLLALGCVDVNRRPGVDGATPLHCAASGGSRNAVAVVKLLLGAGADPVTPDSAGRLPADVILSPPASPDALGDLEMLLGRRRGLAVATSVPSLSSSPPLSSSPDESNRSPSSRSSSLSPITVDRAKKEYPVDPTLPDIKSRVYASDEFRMFAFKVRPCSRAYSHDWTECPFVHPGENARRRDPRKHPYTAVPCPNFRRPGGCPSGDSCEFSHGVFESWLHPSQYRTRLCKEGAACARRICFFAHDEDELRHVPHNSGAGLLSPRASSSIDMTAAAAALGLLPGSPTRHFVPPPLSPSAANNGGGAAAHWLQGSRLRSSFNARDAQADDLGSLLEWESQYLGALSLPQSSRSQPRLSTGLTIRPTAVAPSYLEEMYASDMAMSPRFTNDQGHSVFSPAHKSALLNKFHHQQKGLLSPVNTNRMYSPRGLDPSIIHSPFGGMSPRSPRTMELTSPLSVRVGVGAAVTPRDMLDQFSSLNKHQVPSVGSPRNLNASWGNIGTPKSKVDWGVDDDELVRLRHPVQHGNTEDEPDVSWVQSLVNHAELNGKRGEMASMASPSINKPDLSF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCGACCTTGCTGATCTCGCGGGTGCGGGCGAGGCGGCGCGTTCGCATCTCGCCGCCGGCGGCCAGCGGGACAGGCTGGCGGCTCTGCTGGAGCTCGCGGCGGCCGACGACGTCGCGGGTTTCCGCGAGGCGCTCGCGGGGGGAGGCGGCGAGGGCGACACGGCGGAGCTGGCGGACGGAGTGGGGATGTGGTACGGCCGAAGCAAGGCGTATGAGCCGCGCACGCCGCTGATGGTCGCCGCGACTTACGGCAGCGTGGAGGTGGTGTCGCTTCTGCTCGCCCTTGGTTGTGTCGACGTCAACCGGCGCCCCGGCGTCGACGGCGCCACTCCGCTTCACTGCGCCGCCTCCGGCGGCTCGCGGAACGCTGTGGCCGTCGTCAAGCTGCTCCTGGGTGCCGGCGCCGACCCGGTAACTCCCGACTCCGCCGGTCGCTTGCCTGCCGACGTCATCTTGTCTCCACCTGCTTCGCCCGACGCCTTGGGCGATCTGGAGATGCTCCTTGGCCGCCGCCGAGGGCTCGCTGTCGCGACCTCGGTGCCATCGCTCTCGTCGTCCCCGCCGCTCTCGTCCTCGCCGGATGAGAGCAACAGGTCGCCGTCCTCGCGGTCGTCTTCGTTGTCTCCGATTACCGTCGACCGTGCCAAGAAGGAGTACCCGGTGGATCCGACGCTGCCCGACATCAAGAGCCGCGTGTATGCCTCCGACGAGTTCCGCATGTTCGCGTTCAAGGTGCGGCCATGCTCCAGAGCCTACTCGCATGACTGGACAGAGTGTCCGTTCGTGCACCCTGGCGAGAACGCCCGCCGCCGGGATCCTCGCAAGCACCCATACACGGCGGTGCCCTGCCCCAACTTCCGCCGCCCTGGTGGCTGCCCCAGCGGTGATAGCTGCGAGTTCTCCCATGGTGTTTTCGAGAGCTGGCTCCACCCGTCGCAGTACCGCACAAGGCTCTGCAAGGAGGGCGCAGCTTGTGCCCGCCGCATCTGCTTCTTCGCCCACGATGAAGATGAGCTCCGCCATGTGCCTCACAATAGTGGTGCAGGTCTGCTGTCTCCCCGTGCCTCTTCATCCATTGACATGACCGCTGCTGCTGCTGCTCTAGGGCTTCTACCTGGATCTCCTACCAGGCACTTTGTGCCACCACCTCTGTCACCATCTGCTGCCAACAATGGTGGAGGTGCTGCTGCTCATTGGCTCCAAGGTAGCAGGCTGCGCTCTTCTTTCAATGCAAGAGATGCACAGGCTGATGACCTTGGCTCGCTCCTGGAATGGGAATCTCAATACCTTGGAGCGCTCAGCCTCCCACAAAGCAGCCGATCCCAGCCCCGTCTTTCTACTGGTCTGACTATTCGTCCAACAGCAGTTGCTCCATCCTACCTCGAAGAGATGTATGCTTCAGACATGGCTATGTCGCCAAGGTTCACCAATGACCAAGGTCACTCAGTCTTCTCACCAGCACACAAATCAGCTCTTCTAAACAAGTTTCATCATCAACAGAAGGGCCTTTTGTCGCCTGTCAATACCAACAGGATGTACTCACCAAGGGGCCTTGATCCATCAATTATCCATTCTCCATTTGGTGGCATGTCTCCTCGGTCCCCTCGGACTATGGAGCTAACATCTCCCCTAAGTGTCCGTGTTGGTGTTGGAGCTGCTGTTACTCCGCGTGATATGTTGGATCAGTTTTCTTCCCTGAACAAGCACCAGGTGCCATCTGTGGGATCGCCACGGAATCTTAATGCCTCATGGGGCAACATTGGTACCCCAAAGAGTAAGGTCGACTGGGGTGTGGACGACGACGAACTAGTGCGCTTGAGGCACCCTGTGCAACATGGAAATACAGAAGACGAACCAGATGTGTCTTGGGTGCAGTCACTGGTAAACCATGCTGAGCTGAATGGCAAGAGGGGTGAAATGGCAAGCATGGCTTCTCCATCGATTAACAAGCCTGACCTGAGCTTT
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