| TFome pUT3376 | |
|---|---|
| Template | BT054532 |
| Genbank Insert ID | KJ726836 |
| Protein Name | ZmTCP10 |
| Species | Zea mays |
| TF Family | TCP |
| Gene id | GRMZM2G142751 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G35A p.R12H. In template FlcDNA. Not in functional domain. c.A407C p.Y136S. In template FlcDNA. In functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3376
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2716up |
| Temperature (ºC) | 61.5 |
| Sequence
|
caccATGGACGCCACCGGAGATGC |
3' Primer
| Name | UT2716dn |
| Temperature (ºC) | |
| Sequence
|
CGAGTCGTCGTCGCTGGGG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDATGDAGGGRHPNFPLQLLEKKEEQPCSTSPAAAVGAGANGSGEMQMKKVAPPKRTSSKDRHTKVDGRGRRIRMPAICAARVFQLTRELGHRTDGETIEWLLQQAEPAVIAATGTGTIPANFTSLNISLRSSGSSFSIPAHLRAAGLPGPRFSGARGDPWERVVGLGFGGAEGPPSATSSPLLLSFHSGSVGLDVSSPSSSSAAANSDLSKKRRWEQEMQQQQQQYQQQMAGYTQSQMPGTVWMVPSSNAQAAAAAGGGGSSNNESIWTFPPQPGSGDGGAAPVYRGVPSGLHFMNFPAPMALIPGGQQLGIGHENGGSSSGGEGHMGVLAALNAYRAHAVTVAAPAAQNGAEGGSSQQQQRGGGERQDSMSPSDDDS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGCCACCGGAGATGCCGGCGGAGGTCGCCACCCCAACTTCCCTTTGCAGCTTCTGGAGAAGAAGGAGGAACAGCCTTGCTCCACGTCCCCAGCAGCTGCTGTGGGTGCTGGGGCTAATGGGTCGGGAGAGATGCAGATGAAGAAGGTGGCGCCGCCGAAACGCACATCGAGCAAGGACCGGCACACGAAGGTGGACGGGCGCGGGCGGCGCATCCGGATGCCGGCAATCTGCGCAGCTCGGGTGTTCCAGCTGACGAGGGAGCTGGGGCACAGGACGGACGGCGAGACAATCGAGTGGCTGCTGCAGCAGGCGGAGCCCGCGGTGATCGCGGCCACGGGCACCGGCACCATCCCGGCCAACTTCACCTCGCTCAACATTTCTCTCCGCTCCTCGGGCTCCTCCTTCTCCATCCCAGCGCACCTCCGTGCTGCCGGTTTGCCCGGCCCACGCTTCAGCGGCGCGCGGGGAGACCCGTGGGAGCGCGTCGTGGGCCTCGGGTTCGGCGGCGCCGAGGGTCCTCCATCTGCTACTTCGTCTCCGCTGCTGCTAAGCTTCCACTCCGGCAGCGTCGGCCTTGACGTGTCATCACCGTCGTCGTCGTCTGCTGCCGCCAACTCCGACCTGTCGAAGAAACGGCGGTGGGAGCAAGAAATGCAGCAGCAGCAGCAGCAATACCAGCAGCAGATGGCCGGGTACACGCAGAGCCAAATGCCAGGAACCGTGTGGATGGTGCCGAGCAGCAACGCTCAGGCCGCAGCAGCGGCTGGCGGAGGCGGCAGCAGCAACAACGAGTCGATCTGGACGTTCCCGCCGCAACCAGGAAGCGGCGACGGCGGCGCGGCGCCCGTGTACCGCGGCGTGCCGAGTGGGCTACATTTCATGAATTTCCCCGCCCCGATGGCGCTGATACCCGGTGGGCAGCAGCTCGGGATCGGCCACGAAAATGGCGGCAGCAGCAGCGGAGGCGAGGGCCACATGGGGGTCCTCGCCGCGCTCAACGCGTACCGGGCGCATGCCGTCACGGTGGCTGCGCCGGCGGCGCAGAACGGAGCGGAAGGCGGCTCTAGTCAGCAGCAGCAGCGTGGCGGCGGCGAGCGACAAGACAGCATGAGCCCCAGCGACGACGACTCG
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