| TFome pUT3382 | |
|---|---|
| Template | BT061116 |
| Genbank Insert ID | KJ726842 |
| Protein Name | ZmCOL5 |
| Species | Zea mays |
| TF Family | C2C2-CO-like |
| Gene id | GRMZM2G075562 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3382
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2808up |
| Temperature (ºC) | 55.6 |
| Sequence
|
caccATGGCTTCTCTCTGTGATTTCTGTG |
3' Primer
| Name | UT2808dn |
| Temperature (ºC) | |
| Sequence
|
GTAGCTTCTAGTCTGGCTTAGCGG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MASLCDFCGKQRSMIYCRSDAASLCLSCDRNVHSANALSRRHTRTLLCDRCGSQPASVRCLEDNASLCQNCDWNGHDAESGASGHKRQAINCYSGCPSSAELSRIWSFITDIPTVAAEPDYEDGLSMMTIDGSDVTNRHDTSDDKRLLEIANTTLMSDPPSADKLKSPTGSSSGDGFDVMTLATDQPAGPVSATPKVPNARDDDKFNDGMYEDLCVDDADLTFEDYEELFGTSHIRTEELFDDAGIDGYFELKETPPFYFNEQPKAMQIECGNVVSADCAMSNPGARADSSLCIPVRQVRSSISHSLSGLTGESSAGDHHDCGVSPMLLMGEPPWHSPGGPEGSVAGGSRDSALTRYKEKKKRRKFDKKIRYASRKARADVRKRVKGRFIKAGEAYDYDPLSQTRSY
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTTCTCTCTGTGATTTCTGTGGGAAACAAAGGTCAATGATCTACTGCAGATCAGATGCGGCATCATTGTGCTTATCATGCGACCGTAATGTCCATTCAGCTAATGCACTGTCTCGACGTCATACAAGGACCCTTCTTTGTGATCGTTGTGGTTCACAGCCTGCATCAGTCCGATGTCTTGAGGACAACGCATCACTTTGCCAAAACTGTGACTGGAATGGGCATGATGCAGAATCAGGGGCTTCTGGACATAAAAGGCAAGCCATAAACTGCTACTCAGGGTGCCCATCATCTGCAGAGCTTTCGAGAATCTGGTCATTTATTACTGATATCCCGACTGTAGCAGCTGAGCCCGACTATGAGGATGGACTAAGCATGATGACAATTGATGGCAGTGATGTTACTAATCGTCACGATACTTCAGATGATAAAAGATTGTTGGAAATAGCTAACACAACACTCATGAGCGATCCACCTTCAGCCGACAAGCTTAAATCTCCGACAGGCTCTTCTTCTGGAGATGGGTTTGATGTTATGACTCTAGCCACAGATCAGCCTGCTGGACCAGTTTCAGCAACGCCTAAGGTACCTAATGCCAGAGATGATGATAAGTTCAATGATGGCATGTACGAAGACTTGTGTGTAGATGATGCTGACCTTACATTCGAGGATTATGAAGAGCTATTTGGTACCTCTCACATTCGAACAGAGGAACTCTTTGATGACGCTGGAATTGACGGTTACTTCGAATTGAAAGAGACACCACCTTTTTATTTCAACGAGCAGCCCAAAGCTATGCAGATAGAATGTGGCAACGTGGTATCAGCTGATTGTGCGATGTCAAACCCAGGGGCAAGGGCTGATTCCAGCCTTTGTATTCCCGTCAGGCAGGTCAGATCTAGTATATCCCACTCCTTATCTGGTCTGACTGGTGAGAGCAGCGCTGGAGATCACCACGACTGTGGGGTGTCACCGATGCTCCTCATGGGTGAGCCACCCTGGCATTCTCCTGGTGGTCCAGAAGGCTCAGTCGCTGGAGGCAGCAGAGATAGCGCTCTCACACGGTACAAGGAGAAGAAGAAGAGGAGAAAGTTTGACAAGAAGATCAGATATGCTTCTCGCAAGGCTAGGGCGGACGTGAGGAAGCGGGTCAAGGGACGGTTCATAAAGGCCGGTGAAGCGTACGACTATGATCCGCTAAGCCAGACTAGAAGCTAC
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