TFome clone pUT3394

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT2777up
Temperature (ºC)	59.0
Sequence Copy to clipboard	caccATGGGGGAAGGCACAGAGAGC

3' Primer

Name	UT2777dn
Temperature (ºC)
Sequence Copy to clipboard	AACCGACAGAAGCCATCTCAAATATTC

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MGEGTESGGGATTAPPLRYIRCYKCGFCDVVRSKKCLLRAHVLEHHKDEVDALGGYWEGGDAGPRKEISRACEQCGMSFKKQAHLKQHMQSHSLERPFSCTVDGCPFSYSRKDHLNRHLLTHEGKLFVCPVEGCGRKFNIKGNMQRHVQEIHKDVSPCESKKEFICPKVNCGKAFKYASKLKKHEESHVQLEYTEVMCCEPGCMKFFSNMECLKAHNQSCHQYVQCDICGTKQLKKNFQRHHRMHEGSCVTERVKCHIEDCKCSFSKKSNLDKHVKAVHEQRRPFVCQFSGCGKRFSYKHVRDNHEKSSAHVHTEGDFVEADEQRPRSVGGCKRKPVSVESLMRKRVAAPDDGPAHADATEYLRWLLSV

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGGAAGGCACAGAGAGCGGCGGCGGGGCGACCACAGCGCCGCCTCTGAGGTATATAAGGTGCTACAAGTGCGGGTTCTGCGACGTGGTCCGGTCCAAGAAGTGCTTGCTCCGCGCCCACGTGCTTGAGCACCACAAGGATGAGGTGGATGCTTTGGGGGGTTACTGGGAAGGTGGAGATGCTGGTCCGCGCAAAGAGATCAGCCGTGCGTGCGAACAGTGCGGGATGAGCTTCAAGAAGCAGGCGCATCTGAAGCAACATATGCAGAGCCATTCGCTCGAGAGGCCCTTTTCCTGCACTGTTGATGGTTGCCCCTTTAGCTATAGCAGGAAGGATCATTTGAACAGACATTTACTTACTCATGAAGGGAAACTATTTGTGTGCCCTGTCGAAGGATGTGGCCGTAAGTTCAATATCAAGGGTAATATGCAGAGACATGTTCAGGAAATCCACAAAGATGTCTCTCCTTGTGAAAGCAAGAAAGAATTCATCTGTCCAAAGGTTAACTGTGGGAAGGCTTTCAAATATGCTTCTAAGTTAAAGAAGCACGAAGAATCACATGTCCAGCTGGAATACACAGAAGTTATGTGCTGTGAGCCAGGCTGCATGAAGTTCTTTTCAAACATGGAATGCCTGAAGGCGCATAACCAATCTTGCCATCAATATGTTCAGTGTGATATCTGTGGTACGAAACAGCTAAAGAAGAATTTCCAGCGCCATCATCGGATGCATGAAGGTTCCTGCGTCACTGAGAGGGTTAAATGCCACATCGAGGACTGCAAATGTTCGTTCTCGAAGAAATCCAATTTGGACAAGCATGTTAAGGCGGTACATGAGCAGCGTCGTCCTTTTGTGTGCCAATTCTCTGGGTGTGGCAAGAGGTTTTCTTACAAGCACGTAAGGGACAATCATGAGAAGTCAAGCGCTCATGTGCACACTGAGGGTGATTTTGTCGAGGCTGATGAGCAGCGGCCACGTTCAGTAGGTGGGTGCAAGAGGAAACCCGTATCTGTTGAGAGTTTGATGCGTAAGAGGGTAGCCGCTCCTGACGATGGGCCTGCTCATGCTGATGCAACTGAATATTTGAGATGGCTTCTGTCGGTT