| TFome pUT3418 | |
|---|---|
| Template | BT067085 |
| Genbank Insert ID | KJ726877 |
| Protein Name | ZmGRF1 |
| Species | Zea mays |
| TF Family | GRF |
| Gene id | GRMZM2G178261 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3418
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2854up |
| Temperature (ºC) | 60.0 |
| Sequence
|
caccATGGACCTGGGCGGGTCG |
3' Primer
| Name | UT2854dn |
| Temperature (ºC) | |
| Sequence
|
CACAAGGCGGATGCTTGGATG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDLGGSVVMDAGASELGLLGGGSSRLLKHGRGPNAAGGDDDVWGGGRAKQPRTGTAPVVAGDVAAATKAAAAPFLLGSCSPGHGGEQMLSFSSAAAAAAASCASSTAAADGGGAMPLYYGTPASCSGLSSVSLSTSMQGAMARVRAPFTPSQWIELEHQALIYKYLAANSPIPHSLLIPIRRSLAASPYPPSYFGTSTLGWGSFQLGYSGNADLEPGRCRRTDGKKWRCSRDAVADQKYCERHMNRGRHRSRKHVEGQPGHAAKAMSAVAAAAAAAAQPAALAGAGAPAAGLTVNQHQLPAKSYATGATDPCSLQYNRELVNKQNESENMQDSDNLSMLTSMNTGNTGSVFPFSKQNTNPFEVTSSRLEFGLVSSDSLMSSPHSSLENVNLLGSQSMNEHQNPASLQHFVDWPRTPAQGGLSWPEAEDMQAQRSQLSISAPMASSELSSASTSPIHEKLMLSPLKLSREYSPTGLGIAANRDEASQLEATWATMFRDSSMGGPLGEVLAKNGNVEARNYLSAPLNLLTDYWDSSHGMESSPVGVLQKTTAFGSVSSSTGSSPRVENHGAYDGFGNLRDDLGSIVVRHPSIRLV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACCTGGGCGGGTCGGTGGTGATGGACGCGGGCGCCTCGGAGCTGGGCCTGCTCGGCGGCGGATCTAGCAGGCTGCTCAAGCACGGGCGGGGGCCCAATGCGGCGGGGGGAGACGACGACGTGTGGGGCGGCGGCAGGGCCAAGCAGCCCAGGACAGGGACAGCGCCCGTCGTCGCCGGGGACGTGGCGGCTGCCACCAAGGCGGCGGCGGCGCCGTTCCTGCTGGGCTCGTGCAGTCCCGGCCACGGCGGGGAGCAGATGCTCAGCTTCTCGTCCGCCGCCGCCGCCGCCGCGGCCTCGTGCGCCTCCTCCACCGCTGCGGCTGATGGCGGCGGGGCCATGCCGCTGTACTACGGGACCCCGGCCTCTTGCTCAGGATTGAGCTCGGTGAGCTTGAGCACCAGCATGCAGGGGGCAATGGCCAGGGTGAGGGCCCCATTCACGCCGTCCCAGTGGATCGAGCTCGAACACCAGGCCCTGATCTACAAGTACCTGGCCGCGAACAGCCCCATACCTCACAGCCTCCTCATCCCCATCAGGAGGAGCCTCGCAGCGTCTCCCTACCCGCCTTCATACTTCGGCACGAGCACATTGGGGTGGGGCTCTTTCCAGCTGGGCTACTCCGGCAATGCGGATCTGGAGCCTGGGCGGTGCCGCCGGACGGACGGCAAGAAATGGCGGTGCTCCAGGGACGCGGTCGCCGACCAGAAGTACTGCGAGCGACACATGAACCGGGGACGCCATCGTTCAAGAAAGCATGTGGAAGGCCAGCCTGGCCATGCCGCGAAAGCCATGTCCGCGGTGGCGGCGGCGGCTGCTGCTGCTGCCCAGCCTGCTGCTCTGGCCGGCGCCGGAGCTCCTGCTGCTGGCCTCACTGTCAATCAGCACCAGCTGCCGGCGAAGAGCTACGCCACGGGTGCGACTGACCCTTGCTCTCTGCAATATAACAGGGAACTGGTGAACAAACAGAATGAGAGCGAAAACATGCAGGACTCCGACAACCTCTCGATGCTGACTTCCATGAACACAGGAAACACAGGCAGCGTGTTCCCATTCTCGAAACAAAACACCAATCCTTTTGAAGTGACCAGCTCAAGGCTAGAGTTCGGCCTCGTTTCATCGGATTCACTGATGAGTTCCCCTCACAGTTCCTTGGAGAACGTCAACCTGCTCGGCTCACAGAGTATGAACGAACACCAGAACCCAGCCTCCCTGCAACACTTCGTGGACTGGCCCAGGACGCCTGCCCAGGGAGGCCTTTCATGGCCCGAAGCTGAAGACATGCAGGCTCAGAGGTCACAGCTCTCCATATCTGCTCCCATGGCATCCTCCGAGCTATCGTCGGCCTCCACATCCCCCATCCACGAGAAGCTGATGCTGTCGCCTCTGAAGCTGAGCCGCGAGTACAGCCCGACTGGTCTCGGCATTGCGGCGAACAGAGACGAGGCTAGCCAGCTAGAAGCAACCTGGGCGACGATGTTCCGTGACTCTTCGATGGGCGGTCCGTTGGGGGAGGTTCTGGCCAAGAACGGCAACGTGGAAGCGAGAAATTACCTGTCGGCGCCTCTGAACCTTCTCACCGACTACTGGGATTCAAGCCACGGGATGGAGTCCTCCCCTGTGGGCGTTCTGCAGAAGACGACCGCCTTCGGATCGGTCTCCAGCAGTACAGGGAGCAGCCCTAGGGTTGAGAACCACGGCGCCTACGACGGTTTCGGCAACCTGCGGGATGATCTCGGCTCAATTGTAGTGCGCCATCCAAGCATCCGCCTTGTG
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