| TFome pUT3422 | |
|---|---|
| Template | BT067328.1 |
| Genbank Insert ID | KJ726881 |
| Protein Name | ZmGRAS19 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM2G172657 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3422
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2929up |
| Temperature (ºC) | 59.0 |
| Sequence
|
caccATGGATACGCTGTTTAGGTTGGTTAGC |
3' Primer
| Name | UT-2929dn |
| Temperature (ºC) | |
| Sequence
|
TGGCCTCCACGCGCTCG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDTLFRLVSLQASEQQQQQSASYNSRSTTSSGSRSSSHQTNASYNYYYHSNSSGGGGGQYYYGQQHPHQHQHQQYYLEPYQQEECGNTHHLYMDEDFSSSSSSRQHFHSHGAVVQPPTSSTATPTAPTPSLSTSSTAAGAAHALFEAADLSFPPDLNLDFSSPASSSGGGAASSAAVGGGGGGRWASQLLLECARAVAGRDSQRVQQLMWMLNELASPYGDVEQKLASYFLQGLFARLTASGPRTLRTLAAASDRNTSFDSTRRTALRFQELSPWSSFGHVAANGAILESFLEAAAASPEPQRLHILDLSNTFCTQWPTLLEALATRSADDTPHLSITTVVSSAPSAPTAAVQRVMREIGQRMEKFARLMGVPFSFRAVHHAGDLAGLDLDALDLRDGGATTALAINCVNSLRGVVPGGARRRDAFAASLRRLDPRVVTVVEEEADLVAFDPGAPEESGDTEAAFLKVFGEGLRFFSAYMDSLEESFPKTSNERLALERGAGRAIVDLVSCPASESMERRETAASWARRMRSSGFSPVAFSEDVADDVRSLLRRYREGWSMRDAGLDDSAAGAGVFLAWKEQPLVWASAWRP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATACGCTGTTTAGGTTGGTTAGCCTTCAAGCCTCCGAGCAGCAGCAGCAGCAATCGGCGTCCTACAACTCGAGGAGCACCACGTCGAGCGGCTCCAGGTCGTCGTCGCACCAGACCAACGCATCCTACAACTACTACTACCACAGCAACAGCAGCGGCGGCGGCGGCGGGCAGTACTACTACGGTCAGCAGCACCCACACCAGCACCAGCACCAGCAGTACTACCTGGAGCCGTACCAGCAAGAAGAATGCGGCAACACCCACCACCTTTACATGGATGAAGACTTCTCCTCCTCGTCCTCGTCGAGGCAGCATTTCCACTCGCACGGCGCGGTGGTGCAGCCGCCGACGTCGTCCACGGCCACGCCCACGGCGCCGACGCCCTCGCTGTCCACGTCGTCCACGGCCGCGGGGGCGGCGCACGCGCTGTTCGAGGCGGCCGACCTGTCGTTCCCGCCTGACCTCAACCTCGACTTCTCGTCCCCGGCCTCGTCGTCCGGCGGGGGCGCGGCCTCGTCGGCGGCGGTCGGGGGAGGTGGCGGGGGAAGGTGGGCGAGCCAGCTGCTGCTGGAGTGCGCGCGCGCGGTGGCGGGCCGCGACAGCCAGCGCGTGCAGCAGCTGATGTGGATGCTCAACGAGCTGGCCTCGCCGTACGGGGACGTCGAGCAGAAGCTGGCGTCCTACTTCCTCCAGGGGCTCTTCGCGCGCCTCACGGCGTCCGGACCGCGGACGCTGCGCACGCTCGCTGCGGCGTCCGACCGGAACACGTCCTTCGACTCCACGCGGCGCACGGCGCTGCGGTTCCAGGAGCTCAGCCCGTGGTCGTCGTTTGGGCACGTGGCCGCCAACGGCGCCATCCTGGAGTCGTTCCTGGAGGCCGCCGCGGCGTCGCCGGAGCCCCAGAGGCTCCACATCCTCGACCTCAGCAACACGTTCTGCACGCAGTGGCCCACGCTGCTCGAGGCGCTCGCCACGCGGTCCGCCGACGACACGCCGCACCTGTCGATCACCACGGTGGTCTCCTCCGCGCCGTCCGCGCCGACGGCCGCCGTGCAGCGCGTGATGCGGGAGATCGGGCAGCGGATGGAAAAGTTCGCGCGGCTGATGGGCGTGCCCTTCAGCTTCCGCGCAGTGCACCACGCCGGGGACCTTGCGGGGCTCGACCTCGACGCGCTCGACCTGCGCGACGGCGGCGCCACCACCGCGCTCGCCATCAACTGCGTCAACTCGCTGCGCGGCGTGGTGCCGGGCGGTGCGCGGAGACGGGACGCGTTCGCCGCGTCCCTCCGGCGTCTCGATCCGCGGGTTGTTACTGTCGTCGAGGAGGAGGCCGATCTCGTGGCTTTTGACCCCGGCGCGCCCGAGGAAAGCGGCGACACGGAGGCAGCGTTCCTGAAGGTGTTCGGCGAGGGCTTGCGGTTCTTCTCGGCTTACATGGACTCGCTGGAAGAGAGCTTCCCCAAGACTAGCAACGAGAGGCTGGCGCTGGAGAGGGGAGCCGGACGTGCCATTGTAGACTTGGTCTCGTGCCCGGCGTCGGAGTCCATGGAGCGGCGGGAGACGGCGGCTTCATGGGCGCGCCGCATGCGGTCTTCCGGCTTCTCTCCGGTGGCGTTCAGCGAGGACGTCGCCGACGACGTGCGGTCGTTGCTCCGTCGGTATCGGGAAGGCTGGTCGATGCGGGACGCCGGTTTAGACGACTCGGCAGCCGGAGCAGGCGTCTTCCTGGCGTGGAAGGAACAGCCTCTCGTGTGGGCGAGCGCGTGGAGGCCA
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