| TFome pUT3436 | |
|---|---|
| Template | DT940843 |
| Genbank Insert ID | KJ726893 |
| Protein Name | ZmC3H14 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM2G150262 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T1747C p.C583R. Not in functional domain. SNP not covered by EST. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3436
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2587up |
| Temperature (ºC) | 69.1 |
| Sequence
|
caccATGGAGGTGGCGGCGGCTG |
3' Primer
| Name | UT-2587dn |
| Temperature (ºC) | |
| Sequence
|
AGGCAATAAAACCCCTACTTTCCGAGC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MEVAAAAAGKPLTPEEEALRRSTDCVYFLASPLTCKKGNECDFRHSEGARMNPRDCWYWLNGSCLNPKCAFRHPPIDGLFGAPTSGLPPVSAHYGAYNLGKQMVPCYYFQKGNCLKGDRCPFYHGPQTASNDLAEQAAKVSSFPLEPSQAQNNEEAVAPNNSTQQEARMTENRTSIHVSKSGVGATPADVASNALKPGTNFEQAPSNTLAAKKSSTTEDHPMHYQNQVPVEIDPVKDWNQNFEVSPTDYLPQDSREADDILGESSPGFDVLVDNDVDVAEDFARDMYPVEDYEYVTSDFDARAHHESEQFNDGMGVNGRIGQYDGYERKRRRSSSERNLDRHYHPDGRFLHRELDRGEIDGSDLRHQLRRRRINGPSTTISPERANGDRHGRDEPYRERAHGGHHTYRDRYQGPRGSNLSSRLQARIKLPRRSPDRVDTRFEDERDRRRFRDMFSPMRRMDFHGGRHQEAGHNQERGHRKLSELVSTVRHADGLSARRDAVGSTHFAARRNLGEPRKANGLVESEASLDFEGPKPLSVILQRKREAAQGNNYEKFAEVAVTQTGSLDETEKKSCDNVTRFADRKSGSGDEEYKEQDHILVDAHRQSSDHGDKFEAGYAAEVDAEGKQEADDYDQREGESDDYETFEGHDYKSEDENVYQDDEDFDDNDDFARKVGVLLP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGTGGCGGCGGCTGCCGCAGGGAAGCCGCTGACGCCGGAGGAGGAGGCACTCCGGCGCAGCACCGACTGCGTCTACTTCCTCGCCTCGCCGCTCACCTGCAAGAAGGGTAATGAATGCGATTTTCGTCACAGTGAGGGTGCTAGGATGAACCCTAGGGACTGTTGGTACTGGTTGAATGGCAGTTGTTTGAATCCCAAGTGTGCATTTCGCCATCCGCCAATTGATGGTCTCTTTGGTGCCCCAACTTCTGGACTACCTCCAGTTTCTGCGCACTACGGTGCCTATAACTTGGGCAAGCAGATGGTCCCATGCTATTACTTCCAGAAAGGGAACTGCTTGAAGGGTGATAGATGCCCTTTCTATCATGGACCACAAACAGCTAGTAATGATCTTGCAGAGCAAGCGGCGAAGGTTTCTTCCTTTCCTTTGGAACCGTCCCAGGCTCAAAATAACGAAGAGGCTGTAGCTCCAAATAATTCAACGCAGCAAGAGGCCCGAATGACTGAAAACAGGACTTCGATTCATGTTTCCAAGAGTGGTGTGGGTGCAACTCCCGCTGATGTAGCTTCCAATGCATTGAAGCCTGGGACCAACTTCGAGCAGGCACCCAGTAATACGCTTGCAGCAAAGAAAAGTTCCACAACTGAGGATCATCCTATGCACTATCAGAACCAGGTTCCTGTGGAGATCGATCCTGTCAAAGACTGGAACCAAAATTTTGAAGTGTCTCCTACTGATTACCTGCCACAGGATAGTAGGGAGGCTGATGATATTTTGGGGGAGTCCTCTCCTGGTTTTGATGTTCTTGTAGACAATGATGTAGATGTTGCTGAAGATTTTGCAAGGGATATGTACCCGGTAGAAGATTACGAGTATGTTACATCTGATTTTGATGCTCGGGCCCATCATGAAAGTGAGCAGTTCAACGATGGAATGGGTGTGAATGGACGGATTGGACAGTATGATGGCTATGAGAGGAAACGCCGTAGATCTTCATCTGAGAGGAACCTAGACAGACATTACCATCCAGATGGAAGGTTTCTTCACAGAGAGCTTGATCGTGGCGAGATAGATGGATCAGATCTACGCCATCAGCTCCGCAGGAGAAGAATAAATGGGCCCTCAACAACCATTAGCCCAGAACGGGCTAATGGGGACCGACATGGGAGGGATGAACCCTACAGAGAAAGGGCACATGGTGGTCACCACACGTACAGAGATCGCTATCAGGGCCCACGAGGAAGCAATCTAAGTTCCCGTCTGCAGGCCAGAATAAAGCTTCCTCGAAGATCACCTGATAGAGTTGATACTCGCTTCGAGGATGAGCGAGACAGGAGAAGATTCAGGGATATGTTTTCTCCAATGAGGCGTATGGATTTCCATGGTGGTAGGCATCAAGAGGCTGGACATAATCAAGAAAGGGGCCACAGGAAGTTAAGCGAGCTTGTCTCTACTGTCAGGCATGCAGATGGCCTCTCTGCTAGAAGAGATGCAGTGGGTTCAACTCATTTTGCTGCTCGCAGAAACTTGGGAGAGCCAAGGAAGGCAAATGGTCTTGTGGAATCCGAAGCATCTCTTGATTTTGAGGGCCCAAAACCCCTTAGTGTCATCTTACAGAGAAAAAGGGAGGCAGCACAGGGCAATAACTATGAGAAATTTGCTGAGGTTGCAGTCACGCAGACAGGTTCTCTGGATGAAACTGAGAAGAAAAGCTGTGACAACGTTACCAGGTTTGCAGATCGCAAGAGTGGTTCAGGTGACGAAGAGTATAAAGAGCAAGATCATATCCTTGTGGATGCACACAGGCAGTCTTCGGATCATGGTGATAAGTTTGAGGCTGGGTATGCTGCTGAAGTGGATGCAGAAGGAAAACAAGAGGCAGATGACTATGACCAGAGAGAGGGCGAGTCCGACGACTACGAGACATTTGAAGGCCATGACTACAAATCTGAAGATGAAAATGTGTACCAAGACGACGAGGATTTCGATGATAATGATGACTTTGCTCGGAAAGTAGGGGTTTTATTGCCT
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