| TFome pUT3450 | |
|---|---|
| Template | BT062059.1 |
| Genbank Insert ID | KJ726905 |
| Protein Name | ZmMYBR27 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G095239 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3450
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B338_F |
| Temperature (ºC) | 66.5 |
| Sequence
|
caccATGGGGGCTCCAAAACA |
3' Primer
| Name | B338_R |
| Temperature (ºC) | |
| Sequence
|
AATGTGATAGGATATAAAACATGGTAGTATGTA |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MGAPKQRWTSEEEAALRAGIARHGVGKWRTILKDPEFSSTLCYRSNVDLKDKWRNMNVIVSTSSSRDKAKSALKRIRTIPKNNEHTMAITRVTSDIDDEIVDEKPIVSLPSEAKNTSSSKKSHRSNIGHLVILITYYLQN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGGCTCCAAAACAAAGGTGGACTTCTGAGGAAGAGGCTGCTCTTAGAGCTGGAATAGCAAGGCATGGAGTTGGAAAATGGCGCACAATATTGAAAGATCCAGAATTTAGTTCCACCTTATGCTACCGCTCAAATGTTGATCTCAAGGACAAGTGGCGAAACATGAATGTGATTGTCAGTACATCGAGTTCTCGTGACAAGGCGAAGTCTGCATTGAAGAGAATACGAACTATTCCCAAGAATAATGAGCACACCATGGCAATTACCAGAGTTACTTCTGATATTGATGATGAGATTGTTGACGAAAAGCCTATAGTATCATTGCCTAGTGAAGCAAAGAACACATCAAGTTCGAAGAAATCTCACAGGAGCAATATTGGCCACCTAGTGATTTTGATCACTTACTATCTGCAAAAC
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