| TFome pUT3454 | |
|---|---|
| Template | BT065185.1 |
| Genbank Insert ID | KJ726909 |
| Protein Name | ZmEREB1 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM5G889719 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3454
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B369_F |
| Temperature (ºC) | 69.2 |
| Sequence
|
caccATGGCTCAAGAGCTCCACGAA |
3' Primer
| Name | B369_R |
| Temperature (ºC) | |
| Sequence
|
CTCCCAAAGCAGCGGCTCCT |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MAQELHETSSCSATTTSSCTTSCCSSTVTDSSSSPPSPAAANAAPATRKRQALEAEAEAEAGGEEEEEEEEGCAGNKAAPAKKRPRGSEGKHPTFRGVRMRAWGKWVSEIREPRKKSRIWLGTFPTAEMAARAHDVAALAIKGRAAHLNFPDLAGALPRAASAAPKDVQAAAALAAAFTSPSSEPGAGAHEEPAAKDGAAPEEAAADAQAPVPVALPPPAASRPGTPSSGVEDERQLFDLPDLLLDIRDGFGRFPPMWAPLTDVEEVVNAELRLEEPLLWE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTCAAGAGCTCCACGAAACGTCCTCTTGCTCTGCCACCACCACCTCGTCGTGCACCACATCCTGCTGCTCGTCCACTGTCACAGACTCGTCCTCTTCGCCCCCGTCACCGGCGGCGGCCAATGCCGCGCCCGCGACACGGAAGCGGCAGGCGTTGGAGGCCGAGGCCGAGGCCGAGGCGGGCGGTGAGGAGGAGGAGGAGGAGGAGGAAGGCTGTGCTGGTAATAAGGCGGCGCCGGCCAAGAAGCGACCGCGGGGCAGCGAGGGGAAGCACCCGACGTTCCGCGGCGTGCGGATGCGGGCGTGGGGCAAGTGGGTGTCGGAGATCCGCGAGCCGCGCAAGAAGTCGCGCATATGGCTCGGCACGTTCCCCACCGCCGAGATGGCCGCGCGCGCCCACGACGTCGCGGCGCTCGCCATCAAGGGCCGCGCCGCGCACCTCAACTTCCCGGACCTCGCCGGCGCGCTCCCGCGCGCCGCGTCCGCGGCGCCCAAGGACGTCCAGGCAGCCGCCGCATTGGCCGCTGCGTTCACGTCGCCGTCATCGGAGCCCGGCGCCGGCGCGCACGAGGAGCCCGCTGCCAAGGACGGCGCCGCGCCCGAGGAGGCAGCCGCCGACGCACAGGCACCAGTACCAGTAGCACTACCACCGCCGGCGGCCTCTCGGCCAGGGACGCCGTCGAGCGGCGTGGAGGACGAGCGGCAGCTGTTCGACCTGCCGGACCTGCTCCTCGACATCCGGGACGGGTTCGGGCGCTTCCCGCCGATGTGGGCCCCGCTCACTGACGTGGAGGAGGTGGTCAATGCGGAGCTGCGCCTCGAGGAGCCGCTGCTTTGGGAG
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