| TFome pUT3468 | |
|---|---|
| Template | BT035420.1 |
| Genbank Insert ID | KJ726923 |
| Protein Name | ZmMADS73 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G046885 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A138C p.(=) c.G342A p.(=) c.A375G p.(=) Present in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3468
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B583_F |
| Temperature (ºC) | 69.8 |
| Sequence
|
caccATGGCGAGGGAGAGGCG |
3' Primer
| Name | B583_R |
| Temperature (ºC) | |
| Sequence
|
CTTCCATGCAACGCAAGGCAG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MARERREIKRIESAAARQVTFSKRRRGLFKKAQELSVLCDADVALIVFSSTGKLSQFASSSMNEIIDKYNTHSKNLGKTEQPSLDLNLEHSKYANLNEQLAEASLRLRQMRGEELEGLNVEELQQLEKNLESGLHRVLQTKDQQFLEQINDLERKSTQLAEENMQLRNQVSQIPPAGKQAVADTENVIAEEGQSSESVMTALHSGSSQDNDDGSDVSLKLGLPCVAWK
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGAGGGAGAGGCGGGAGATAAAGAGGATAGAGAGCGCGGCAGCGAGGCAGGTCACGTTCTCCAAGCGCCGCCGCGGCCTCTTCAAGAAGGCCCAGGAGCTCTCCGTGCTGTGCGATGCCGACGTCGCGCTCATCGTCTTCTCCTCCACGGGGAAGCTCTCCCAGTTCGCCAGCTCCAGTATGAATGAGATAATTGACAAGTACAACACGCATTCTAAAAACCTGGGGAAAACAGAACAGCCTTCGCTGGACTTGAACTTAGAGCATAGCAAATATGCAAATTTGAATGAGCAACTTGCGGAAGCAAGCCTTCGACTCAGGCAGATGAGAGGTGAAGAACTTGAGGGATTGAATGTTGAAGAACTCCAGCAGTTGGAGAAGAACCTGGAATCTGGTCTGCATAGGGTGCTTCAAACAAAGGATCAACAATTCTTGGAACAGATCAATGACCTCGAACGTAAGAGTACGCAGCTGGCAGAGGAGAACATGCAACTGAGGAATCAAGTATCCCAGATACCCCCAGCTGGCAAGCAAGCAGTTGCTGATACTGAAAATGTTATTGCTGAAGAAGGGCAATCCTCTGAATCAGTGATGACTGCGTTGCACTCTGGGAGTTCACAGGATAATGATGATGGTTCGGATGTATCTCTAAAGTTAGGGCTGCCTTGCGTTGCATGGAAG
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