| TFome pUT3652 | |
|---|---|
| Template | BT054213.1 |
| Genbank Insert ID | KJ726955 |
| Protein Name | ZmGRAS10 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM2G110579 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3652
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2847up |
| Temperature (ºC) | 67.0 |
| Sequence
|
caccATGGGGGTCGTGGTCGCC |
3' Primer
| Name | UT-2847dn |
| Temperature (ºC) | |
| Sequence
|
GCACCGCCATGCTGATACAGTG |
PCR Condition
Amino Acid Sequence
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MGVVVADSWDAMLGNAAAAAQDQTFLNWFIGAAGDLDQSGPPLPVHHQPLLDNVGFGFPAADPLGFTLDPHLGGVASDMSSPGAVSQTTNSGGGGNKASSAFGLLSPESASFQPPPLPVLFHEGIDTKPPLDTQSPGLLHQYQHQPTPATTFLMSIPSFPSYNQQSPLVHPPAKRHQSIGGDLYLARTRLLPPPPTGQGHAFPPLNGPAPFQLQPSPPLPHGAMKRTAAEAAQQQLLDELAAAAKAAEAGNSIGARAILARLNHQLPPLGKPFLRSASYLKEALLALAEGHHGGCHLKSPLDVALKLAAYKTFSDHSPVLQFTNFTATQALLDEIGGSTSSCIHVIDFDLGVGGQWASFLQELAHCRGAGGAALPFVKLTAFVSAASHHPLELCLARDNIAQFAVDLGIPFEFSAISADMINPAEFISATDDEDVVVVLPTGCSARAPPLPAILGLVKQLAPKIVIAIDHGADRADLPFSQYFLNCFQSCMFLLDSLDAAGIDADSACKIEKFLIQPRIEDSVLGRGKVDNPIAWRSAFAAARFVPVPPSNWAEAEADCLLKRVQVHGFHVEKCGVGLTLYWQHGELVTVSAWRC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGGTCGTGGTCGCCGACAGCTGGGACGCCATGCTCGGCAACGCCGCTGCGGCGGCGCAGGACCAGACGTTCTTGAACTGGTTCATTGGGGCCGCCGGCGACTTGGATCAGTCAGGACCGCCGCTTCCCGTGCACCACCAGCCGCTCCTCGACAATGTGGGCTTCGGGTTCCCGGCAGCGGACCCTCTGGGTTTCACGCTCGATCCCCACCTCGGCGGCGTTGCCTCTGACATGTCGTCCCCTGGTGCGGTGTCGCAAACTACCAACAGTGGCGGCGGTGGCAACAAGGCGTCCTCGGCCTTCGGCCTCTTATCCCCGGAGTCTGCTTCCTTCCAGCCGCCGCCGCTTCCGGTGCTGTTCCATGAAGGCATCGACACAAAGCCCCCTCTTGATACGCAGTCGCCCGGCCTCCTCCACCAGTACCAGCACCAGCCGACCCCCGCCACAACCTTCCTCATGTCCATCCCCTCCTTCCCCAGCTACAATCAGCAGTCGCCACTCGTCCATCCACCGGCCAAACGTCATCAATCCATAGGAGGCGACCTCTATCTCGCCCGAACTCGGCTTCTGCCGCCGCCGCCGACGGGGCAAGGTCATGCCTTTCCACCACTAAATGGTCCTGCTCCATTCCAGCTCCAGCCTTCGCCGCCACTGCCCCACGGGGCAATGAAGAGGACGGCTGCGGAGGCGGCGCAGCAGCAGTTGCTGGACGAGCTGGCGGCGGCGGCAAAGGCCGCTGAAGCTGGCAATTCCATTGGCGCGCGAGCGATATTGGCGCGGCTCAATCACCAGCTTCCCCCGCTCGGGAAGCCGTTCCTCCGCTCCGCCTCCTACCTCAAGGAGGCCCTCCTCGCGCTCGCCGAAGGCCATCACGGCGGCTGCCACCTCAAATCACCGCTCGACGTTGCCCTCAAGCTCGCGGCGTACAAGACTTTCTCTGATCACTCGCCCGTGCTCCAGTTCACCAACTTCACCGCAACGCAGGCACTTCTTGACGAAATTGGTGGCAGCACGTCGTCCTGTATCCATGTCATCGATTTTGATCTTGGCGTTGGAGGCCAGTGGGCTTCTTTCTTGCAGGAACTTGCACACTGCCGTGGCGCAGGTGGCGCAGCTCTGCCGTTCGTTAAGCTCACTGCCTTTGTATCAGCTGCTTCCCACCATCCACTGGAGCTGTGTCTTGCGCGTGACAACATTGCACAGTTTGCTGTTGACCTTGGAATTCCCTTTGAGTTCAGTGCTATCAGTGCTGATATGATCAACCCTGCAGAATTCATTTCTGCCACTGATGATGAAGATGTAGTTGTTGTCCTCCCGACTGGTTGCTCTGCTCGTGCACCACCACTGCCAGCGATCCTTGGGTTGGTGAAACAGCTAGCTCCTAAGATTGTGATAGCCATAGACCATGGCGCTGATCGCGCTGATCTTCCCTTCTCACAGTACTTCTTAAACTGCTTTCAGTCTTGCATGTTCCTCCTTGATTCGCTTGATGCTGCTGGCATTGATGCTGATTCTGCCTGCAAGATTGAGAAGTTCCTGATTCAGCCAAGAATTGAGGATTCGGTGCTTGGGCGGGGCAAGGTGGACAATCCAATTGCATGGAGAAGTGCATTTGCAGCTGCTAGGTTCGTGCCTGTTCCACCCAGCAATTGGGCAGAGGCAGAGGCCGATTGCCTCCTGAAACGGGTGCAGGTCCACGGTTTCCATGTGGAGAAATGTGGGGTCGGGCTCACACTATACTGGCAGCATGGCGAGCTTGTCACTGTATCAGCATGGCGGTGC
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