| TFome pUT3690 | |
|---|---|
| Template | BT039004 |
| Genbank Insert ID | KJ726989 |
| Protein Name | ZmbHLH114 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G095899 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C65T p.P22L Not in functional domain, present in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT3690
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT3297up |
| Temperature (ºC) | 62.6 |
| Sequence
|
caccATGGAGGACTCGAGCCAGTTCATGC |
3' Primer
| Name | UT3297dn |
| Temperature (ºC) | |
| Sequence
|
ACACGTTGGTCCAGCTGCTAGCACT |
PCR Condition
Amino Acid Sequence
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MEDSSQFMQWALSTLQHDELPLATPPAAIAYDDNDCNTFSSVPALGYSAASVNSMVPAEPPAQEGHRATAATNSWSSADTASVTAAQRDAWSPSSQQNSVNCATPRSSGSSQPVSWDFHSASASASAQLIIKEAQVNSATAARAESAAGGMPVPQPQMVQNGSPPTRRASAKMSSASSSAPPCSQDHIVAERKRREKINQRFIELSAVIPCLKKMDKATILSDATRYVKELQEKLKALQQGGSCNARGGTESAPVLVKKPRIAAPGDDDKDRGGAPSPSCAPPGAAATTGNALPEIEARISDGNVVMLRIHCEDGKGVLVRLLAEVEGLRLSITHTNVMPFSACILIINIMAKVAEGFNATADGIVGRLNAVLAAGPTC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGACTCGAGCCAGTTCATGCAGTGGGCCCTGAGCACGCTGCAGCACGATGAGCTCCCACTGGCGACGCCGCCGGCGGCCATCGCCTACGACGACAATGACTGCAACACCTTCTCATCAGTCCCGGCGCTGGGGTACTCCGCTGCATCAGTCAACAGCATGGTCCCGGCGGAACCACCAGCACAGGAAGGCCACCGGGCCACCGCCGCCACCAACAGCTGGAGCTCGGCCGACACCGCGTCCGTCACGGCCGCGCAGCGTGACGCCTGGTCGCCGTCTTCGCAGCAGAACTCGGTCAATTGTGCCACGCCCCGCAGCAGCGGCAGCAGCCAGCCCGTGAGCTGGGACTTCCATTCGGCGTCGGCGTCGGCGTCGGCGCAGCTAATAATCAAAGAAGCCCAGGTCAACTCTGCCACGGCCGCAAGGGCAGAGAGCGCCGCTGGCGGCATGCCGGTGCCACAGCCACAGATGGTTCAAAACGGGTCGCCGCCGACGAGGAGGGCCTCTGCCAAAATGTCGTCGGCTTCTTCGTCTGCGCCGCCGTGCTCCCAGGATCACATCGTCGCGGAGCGGAAACGCCGGGAGAAGATCAACCAGCGTTTCATTGAGCTCTCGGCAGTCATCCCCTGCCTCAAGAAGATGGACAAGGCGACTATCCTATCCGACGCGACGAGGTACGTGAAGGAGCTCCAAGAAAAGCTCAAGGCACTGCAACAAGGCGGAAGCTGCAACGCCCGGGGCGGCACGGAGTCGGCGCCGGTGCTTGTCAAGAAGCCGCGCATAGCGGCGCCGGGGGATGACGACAAGGACCGCGGCGGCGCGCCTTCCCCTTCCTGCGCGCCGCCTGGGGCTGCGGCGACCACCGGGAACGCGCTGCCGGAGATCGAGGCGAGGATCTCGGACGGCAACGTCGTGATGCTGAGGATCCACTGCGAGGACGGCAAGGGGGTGCTCGTCAGGCTGCTGGCCGAGGTCGAGGGACTCCGCCTCAGCATCACGCATACCAATGTCATGCCCTTCTCGGCCTGCATCCTCATCATAAACATAATGGCAAAGGTGGCGGAGGGCTTCAACGCCACGGCGGATGGCATTGTAGGGAGGCTTAATGCAGTGCTAGCAGCTGGACCAACGTGT
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