| TFome pUT3967 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ726995 |
| Protein Name | ZmDOF10 |
| Species | Zea mays |
| TF Family | C2C2-Dof |
| Gene id | GRMZM2G435475 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3967
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | Tup11 |
| Temperature (ºC) | 64.0 |
| Sequence
|
caccATGCTGCCGCCTCACGTCGAG |
3' Primer
| Name | Tdn11 |
| Temperature (ºC) | |
| Sequence
|
GAAAAGCGCGGTCTCCGTTGCAG |
PCR Condition
Step1: 98°C 30s melt, 1 cycle; Step2: 98°C
Amino Acid Sequence
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MLPPHVEMPTTRAAGIKLFGKVITTHYQQQQQPPQPVRHAGAAPASSGRGSGGGPGPDLLEEVARARAAAAEARLPCPRCLSRDTKFCYFNNYNVNQPRHFCRACHRYWTAGGAIRNVPVGSGRRKNRPVLLPPPPPPPHAATATTTGSSATISADDSNDRRRSASGSPPPVFTSAFTAPYYLGSPAQFATSSPAYAAGAAGPPGTAGQCLWLVAATTTTDRSAATETALF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTGCCGCCTCACGTCGAGATGCCGACCACCAGGGCGGCGGGCATCAAGCTGTTCGGCAAGGTCATCACCACGCATTATCAGCAGCAGCAGCAGCCGCCGCAGCCCGTGCGCCACGCGGGCGCCGCGCCGGCGTCGTCGGGGCGCGGGAGCGGCGGCGGCCCCGGCCCCGACTTGCTGGAGGAGGTGGCGCGGGCGCGCGCGGCGGCGGCCGAGGCGCGGCTGCCGTGCCCGCGCTGCTTGAGCCGGGACACCAAGTTCTGCTACTTCAACAACTACAACGTGAACCAGCCGCGCCACTTCTGCCGGGCCTGCCACCGCTACTGGACGGCCGGCGGCGCCATCCGCAACGTGCCGGTCGGCTCCGGCCGCCGCAAGAACCGCCCGGTGCTGCTCCCGCCGCCGCCGCCGCCGCCGCACGCTGCCACCGCCACTACTACCGGCAGTAGTGCTACTATCAGCGCCGACGATAGTAACGACCGCCGCCGCTCGGCCTCGGGGTCTCCTCCTCCGGTGTTTACCTCTGCGTTCACCGCGCCCTACTACCTCGGCTCGCCCGCCCAGTTCGCCACGTCGTCGCCGGCTTACGCCGCTGGCGCTGCCGGCCCGCCGGGGACGGCGGGGCAGTGCTTGTGGCTGGTGGCGGCGACCACGACCACGGATCGTTCTGCTGCAACGGAGACCGCGCTTTTC
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