TFome clone pUT3969

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	Tup14
Temperature (ºC)	59.0
Sequence Copy to clipboard	caccATGATCCAAGAACTGCTCGTCGG

3' Primer

Name	Tdn14
Temperature (ºC)
Sequence Copy to clipboard	GCTAGTGTTGTCTCGTGGGTAAACAGC

PCR Condition

Step1: 98°C 30s melt, 1 cycle; Step2: 98°C

Amino Acid Sequence

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MIQELLVGAAMDQLKRVNDVNPASLPLLLHPVVSNPSPTSSRSSAQQRSTAAATSSPQGQRAEQTPLRCPRCDSSNTKFCYYNNYNLTQPRHFCKTCRRYWTKGGALRNVPIGGGCRRPRPMPTPVATKPAVSCKAVGGAPSLGLGVGLGMGMGTGAGLDGPWASSQQAAAAQAQLMALLNSARGVQGGGSMHRVLGLDAIAHLPLHVLPDAGGSNASGTVPSLWPPRMDSQLGMGPLGQHDVLSSLGRLKLPPPSSAAAASCYSDQLHAVVSSAAGRGHEYEAAAPGVTPLPCTTALASLPPAASSVSAALASATVGLDLPPVSLPASEMQYWAGPAAMSVAWPDLPTPNGVLPRETNPNLMWSCTDDAAVYPRDNTS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGATCCAAGAACTGCTCGTCGGGGCCGCCATGGACCAGCTCAAGAGAGTGAACGACGTGAACCCGGCGTCTCTGCCCCTGCTGCTGCACCCCGTCGTCTCCAACCCGTCGCCCACGTCGTCGCGCTCGTCCGCGCAGCAGCGGTCGACGGCGGCCGCAACGTCGTCGCCGCAGGGGCAAAGAGCGGAGCAGACGCCTCTGCGGTGCCCCCGGTGCGACTCCTCCAACACCAAGTTCTGCTACTACAACAATTACAACCTCACCCAGCCGCGCCACTTCTGCAAGACGTGCCGCCGGTACTGGACCAAGGGCGGCGCGCTCCGCAACGTCCCCATCGGCGGCGGCTGCCGCAGACCGCGCCCCATGCCTACGCCCGTCGCGACGAAGCCCGCGGTCTCCTGCAAGGCCGTGGGCGGCGCGCCGTCCCTGGGCCTCGGCGTCGGCTTGGGCATGGGCATGGGCACGGGCGCCGGCCTCGACGGGCCCTGGGCGTCCTCGCAGCAGGCGGCCGCCGCGCAGGCGCAGCTCATGGCGCTGCTTAACAGCGCCAGGGGCGTGCAGGGCGGCGGAAGCATGCACAGGGTCCTCGGCCTCGACGCCATTGCACACCTGCCCCTCCACGTCCTGCCGGACGCCGGCGGCAGTAATGCCAGTGGCACGGTGCCGTCGCTGTGGCCGCCGCGCATGGACTCGCAGCTCGGCATGGGGCCGCTGGGCCAGCACGACGTGCTGTCAAGCCTCGGGCGGCTAAAGCTGCCCCCACCATCGTCGGCGGCGGCGGCAAGCTGCTACAGCGACCAGCTGCACGCGGTGGTGAGCAGCGCCGCGGGACGCGGACACGAGTACGAAGCTGCAGCCCCCGGCGTCACGCCCCTGCCCTGCACCACAGCGCTGGCCTCCCTCCCTCCAGCCGCGTCGAGCGTGTCGGCTGCGTTGGCCAGCGCCACGGTCGGGCTAGACCTCCCGCCGGTCTCCCTCCCCGCGTCCGAGATGCAGTACTGGGCCGGACCGGCGGCGATGTCCGTGGCGTGGCCGGACTTGCCCACTCCCAACGGCGTGCTCCCTCGAGAGACAAACCCGAACCTGATGTGGTCATGTACGGACGACGCGGCTGTTTACCCACGAGACAACACTAGC