TFome clone pUT5345

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C459F
Temperature (ºC)	61.1
Sequence Copy to clipboard	caccATGCTCCGCCTCCAAAAAC

3' Primer

Name	C459R
Temperature (ºC)
Sequence Copy to clipboard	GATGGCTATTTTCCCATTGCAACC

PCR Condition

Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MLRLQKHLSLRLQEHLLPLHRSASLIQLSLQRSLLSTGSATYSSPGHFAADDYLVSTCGLTREQAANAAKCISHWKSSSNADAVLSFLTGPALGLSNAEIALLVAKDPRVLSCSVDNTLRARLARFRSHGFSAAQISEFVRVAPCFFRKFNIDVKLGFWMPFLGSPDRFLRLVKRNFYLLSSDLDKVVKPNIQLLQECGLSIQEIGSLCVANPRLLTSKPDRIRAVLVRAGEMGVPRKTLLFRHAVTAVAGLCPETFASKLKMMANILGCSEAEVARMVQKNPLVLRRSMETIQRACEFLINVVGVGTNFILDKPTILMYSLERRLVPRHYVMKVLQDKGLMRKDHSFYTLAAISASVFCSRYVHPHKNVLPNLAAAYASGCNGKIAI

TFome clones do not contain stop codon at 3'end.

CDS

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ATGCTCCGCCTCCAAAAACACCTCTCCCTTCGCCTGCAAGAACATCTCCTTCCCCTGCACCGCTCCGCCTCCCTCATACAGCTCTCCCTCCAGCGCTCTCTCCTCTCCACCGGCTCCGCGACATATTCCTCTCCGGGTCACTTCGCCGCCGATGACTACCTCGTCTCCACATGTGGCCTCACCCGGGAGCAAGCCGCGAATGCCGCCAAGTGCATCTCCCACTGGAAGTCGTCCTCCAACGCCGACGCCGTGCTGTCCTTCCTAACCGGCCCGGCGCTCGGCCTCTCCAATGCTGAGATCGCGCTGCTCGTGGCCAAAGACCCGCGCGTCCTCAGCTGCAGCGTCGACAACACCCTGCGGGCCCGCTTGGCCCGCTTCCGCAGTCATGGCTTCTCCGCCGCGCAGATCAGCGAATTCGTCCGCGTGGCCCCCTGCTTCTTTCGCAAATTCAACATCGACGTGAAGCTCGGCTTCTGGATGCCCTTCCTCGGCTCGCCCGACAGGTTCCTCCGCCTCGTCAAGCGTAACTTCTACCTCCTCAGCTCCGACCTCGACAAGGTGGTGAAGCCCAACATCCAGCTGCTCCAGGAGTGCGGTCTATCTATCCAGGAGATTGGGAGCCTGTGCGTGGCTAACCCGAGGCTGCTCACCAGCAAGCCGGACAGAATCAGGGCCGTCCTCGTGCGTGCCGGTGAGATGGGCGTGCCCCGCAAGACGCTTTTGTTCAGGCACGCTGTGACCGCGGTGGCGGGCCTCTGTCCGGAGACCTTTGCATCTAAGCTCAAGATGATGGCCAATATTCTCGGGTGCTCAGAGGCTGAGGTTGCCAGAATGGTGCAAAAGAACCCGTTGGTGCTGAGGCGCTCCATGGAGACGATTCAGCGCGCCTGTGAGTTCCTGATCAATGTGGTCGGTGTCGGTACCAACTTCATTCTGGACAAGCCCACAATCCTGATGTACAGTCTGGAACGCCGGCTGGTGCCCCGGCATTACGTGATGAAGGTGCTCCAGGACAAAGGATTGATGCGAAAAGATCATAGCTTCTACACTTTGGCCGCGATTAGTGCTAGCGTGTTCTGCTCCAGGTATGTACATCCTCATAAGAATGTTCTTCCTAATCTTGCCGCTGCATACGCATCTGGTTGCAATGGGAAAATAGCCATC