| TFome pUT5346 | |
|---|---|
| Template | BT040287 |
| Genbank Insert ID | KJ727487 |
| Protein Name | ZmWRKY15 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G004060 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5346
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C461F |
| Temperature (ºC) | 73.8 |
| Sequence
|
caccATGGCGCCGCCGCCGCC |
3' Primer
| Name | C461R |
| Temperature (ºC) | |
| Sequence
|
CCTACCGTCTCCTACTCTCCTCCAACACACAGCAGGT |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MAPPPPPYARVMEDMVKGREYATQLKALLRESPEASRLVDRILHAISLTIQTASRAWRTWGTTTRSIWKTLCSAPSPAACMLLSTDGRTTTGRPAVCWRRVGDGR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGCCGCCGCCGCCGCCGTACGCGCGGGTGATGGAGGACATGGTCAAGGGCCGGGAGTACGCCACGCAGCTGAAGGCGCTGCTCCGGGAGTCGCCCGAGGCCAGCCGCCTCGTGGACCGGATCCTGCACGCCATATCCCTCACCATCCAGACGGCCTCTCGGGCGTGGCGCACTTGGGGTACGACGACACGTTCGATCTGGAAGACATTGTGTTCGGCTCCATCACCGGCGGCATGTATGCTGCTGAGTACTGACGGCCGGACGACGACTGGTCGACCTGCTGTGTGTTGGAGGAGAGTAGGAGACGGTAGG
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