TFome clone pUT5363

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B490_F
Temperature (ºC)	72.7
Sequence Copy to clipboard	caccATGGCGGGTGGCGGCGCC

3' Primer

Name	B490_R
Temperature (ºC)
Sequence Copy to clipboard	CCTATCGGCAAGAGCTTCAGGATCTGAGAATGTCGTTGA

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence

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MAGGGAGDDDVVVVSCGGRGGGDPGAYAAVLKRKLDLYCAAVAKSMEAKSQEHSLGYPNSQASDASQLISQASFDGDIDGASLVTNSNVIEDDDFQGKPTNSGTSKELSDDDGDLEENTDPANAKKMRRMVSNRESARRSRKRKQAHLTDLESQVSRLTSENASLLKRLADMTQKYKDASVDNKNLTVDVETMRRKVNIAEEAVRRLTGITLMLPTAFEKPTSSAPLSSCPSDAASASASAAIEDSVKHFLQAPLENSGIIPNAAVFQTKPASLHRVASLENLQKRIVGDAMYSETASTTFSDPEALADR

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGGGTGGCGGCGCCGGCGACGACGACGTGGTGGTGGTGAGCTGCGGCGGCAGAGGCGGCGGCGACCCCGGCGCATACGCCGCGGTGCTCAAGAGGAAGCTCGACCTGTACTGCGCCGCCGTCGCCAAATCCATGGAAGCTAAATCACAAGAACATTCGTTGGGCTACCCCAACTCACAAGCTTCAGATGCTTCGCAATTGATTTCTCAAGCTTCCTTTGATGGTGACATAGATGGGGCAAGCCTAGTTACAAACTCTAATGTCATAGAAGATGATGATTTTCAAGGCAAGCCAACCAACAGTGGCACATCAAAGGAACTGTCAGACGATGATGGTGACCTTGAAGAGAATACTGACCCCGCTAACGCCAAGAAGATGAGGAGAATGGTGTCAAACCGGGAATCGGCTAGGCGATCAAGAAAGAGGAAGCAAGCACACTTGACCGATCTTGAATCACAGGTTTCCAGGTTAACGTCTGAGAATGCATCTTTGCTGAAGCGACTGGCTGATATGACTCAGAAATACAAGGACGCTTCCGTTGACAATAAAAATCTAACAGTTGATGTTGAGACTATGAGGAGAAAGGTGAACATAGCCGAGGAAGCTGTCAGGAGGCTAACTGGGATCACCCTCATGCTACCGACCGCTTTTGAGAAGCCCACGAGCAGCGCGCCCCTGTCATCATGTCCATCTGATGCAGCTTCTGCTTCTGCTTCTGCCGCCATTGAAGACAGCGTGAAGCATTTCCTCCAGGCGCCACTTGAAAACAGTGGGATCATCCCAAACGCAGCAGTTTTTCAGACCAAGCCAGCTTCTCTGCACCGTGTTGCTAGCCTGGAGAACCTGCAGAAGAGGATCGTCGGGGATGCAATGTATTCCGAGACCGCGTCAACGACATTCTCAGATCCTGAAGCTCTTGCCGATAGG